›› 2013, Vol. 15 ›› Issue (3): 84-90.DOI: 10.3969/j.issn.1008-0864.2013.03.12

• 生物技术 生命科学 • 上一篇    下一篇

陆地棉LTP家族基因的克隆与表达分析

韩慧超1,黄逸群1,王劲2,左开井1*   

  1. (1.上海交通大学农业与生物学院, 上海 200240|2.中国农科院生物技术研究所 北京 100860)
  • 收稿日期:2012-12-03 修回日期:2013-01-14 出版日期:2013-06-15 发布日期:2013-06-15
  • 通讯作者: 左开井,教授,主要从事棉纤维发育以及植物抗逆的分子生物学研究。E-mail:kjzuo@sjtu.edu.cn
  • 作者简介:韩慧超,硕士研究生,主要从事棉纤维发育的分子生物学研究。E-mail:tiad-06@163.com。
  • 基金资助:

    国家新品种培育转基因重大专项(2011ZX08005-001);国家自然基金项目(31071458)资助。

Cloning and Expression Pattern Analysis of Gossypium hirsutum Lipid Transfer Protein Gene Family

HAN Hui-chao1, HUANG Yi-qun1, WANG Jin2, ZUO Kai-jing1*   

  1. (1.School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240|2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:2012-12-03 Revised:2013-01-14 Online:2013-06-15 Published:2013-06-15

摘要:

长链脂肪酸能够通过调节胚珠内源乙烯信号变化来促进棉花的起始和伸长发育,为深入了解棉花脂肪酸的转运过程及参与基因,对棉花全基因组进行了分析,克隆了11个脂质转运蛋白。实时荧光定量PCR证实:LTP6, LTP7, LTP9, LTP11 4个基因在纤维起始时期(野生型与突变体之间)的表达存在差异,这4个基因可能与纤维起始发育有关。通过基因组步移技术克隆了Gh-LTP6基因的启动子,并对其结构进行分析,发现其具有多个MYB结合位点。这些结果为深入研究LTP基因在纤维起始过程中的角色奠定了基础。

关键词: 陆地棉;LTP家族;基因克隆;棉纤维发育;脂质转运蛋白;启动子

Abstract:

Long-chain fatty acids could activate cotton fiber initiation and elongation by regulating intracellular ethylene signal transduction in cotton ovule. In order to fully understand how fatty acids are transferred to the surface of fiber cells and how many genes are involved in this process, we cloned 11 LTP genes after genome-wide search and analyzed their expression patterns during fibers development by quantitative RT-PCR. The results showed that there are differences between expressions of LTP6, LTP7, LTP9 and LTP11, and these 4 genes might be closely related to cotton fiber development at the original stage. We also cloned Gh-LTP6 promoter by Genome Walking technique and analyzed its motif, results showed there are mutiple MYB binding site. These results might lay a solid foundation for intensive studying the role of LTP genes in the process of cotton fiber development.

Key words: Gossypium hirsutum, LTP family, gene cloning, cotton fiber development, lipid transfer protein, promoter

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