重要养殖鱼类生物育种技术研究

doi:10.13304/j.nykjdb.2022.0711

摘要/Abstract

摘要：

鱼类等水产品作为蓝色食物，被公认为优质的蛋白源，在保障全球食品和营养安全中发挥重要作用。中国是全球最大的水产养殖国家，其成功经验为世界所瞩目。我国在养殖鱼类中建立了选择育种、杂交育种、多倍体育种、性控育种、转基因育种等技术，开展了鱼类基因编辑和全基因组选择育种实践，并在鱼类生殖干细胞和生殖开关等前沿育种技术方面进行了尝试。利用这些技术创制出养殖鱼类新种质，培育出具有优良性状的养殖鱼类新品系和新品种，驱动和支撑着我国水产养殖的高质量发展。系统综述了生物育种技术在鱼类优良种质创制中的开发和应用，并展望了鱼类生物育种技术的发展前景，旨在推动养殖鱼类新品系和新品种培育，支撑我国水产养殖的高质量发展。

关键词: 养殖鱼类, 遗传育种, 生物育种技术, 新品种

Abstract:

As blue food， fish and other aquatic products are recognized as healthy and high-quality sources of animal protein， and play significant roles in supporting global food and nutrition security. China is the largest aquaculture country in the world， and its success stories have attracted global attention. China has developed and utilized various breeding methods for aquaculture species， such as selection breeding， hybridization breeding， polyploid breeding， transgenic breeding， etc. and carried out genome editing and genome selection-based breeding in some species， pioneered some novel breeding methods， such as the fish germline stem cell-oriented approach and reproduction on-off strategy. By using these technologies， Chinese aquaculture scientists have bred various aquaculture species or fish lines with valuable traits， which drives and supports the high-quality development of Chinese aquaculture. This article systematically reviewed the development and application of the above biobreeding techniques in the generation and application of novel fish strains with valuable traits， and provided an outlook on the future development of biobreeding technology， which aimed to promote the breeding of new lines and varieties of aquaculture fish， and support the high-quality development of aquaculture in China.

Key words: aquaculture fish, genetic breeding, biobreeding techniques, new varieties

中图分类号:

孙永华, 胡炜. 重要养殖鱼类生物育种技术研究[J]. 中国农业科技导报, 2022, 24(12): 120-128.

Yonghua SUN, Wei HU. Development of Biobreeding Techniques in Main Aquaculture Fish Species[J]. Journal of Agricultural Science and Technology, 2022, 24(12): 120-128.

参考文献 67

1	GOLDEN C D, KOEHN J Z, SHEPON A, et al.. Aquatic foods to nourish nations [J]. Nature, 2021, 598(7880): 315-320.
2	CRESSEY D. Future Fish [J]. Nature, 2009, 458(7237): 398-400.
3	GUI J, TANG Q, LI Z, et al.. Aquaculture in China: Success Stories and Modern Trends [M]. Wiley-Blackwell, 2018:.
4	NELSON J S, GRANDE T C, WILSON M V. Fishes of the World [M]. John Wiley & Sons, 2016: 1-3.
5	NAKAJIMA T, HUDSON M J, UCHIYAMA J, et al.. Common carp aquaculture in Neolithic China dates back 8,000 years [J]. Nat. Ecol. Evol., 2019, 3(10): 1415-1418.
6	伍献文,钟麟.鲩、青、鲢、鳙的人工繁殖在我国的进展和成就[J].科学通报, 1964, 10: 900-907.
	WU X W, ZHONG L. Progress and achievements of artificial propagation of bighead carp, green carp, silver carp and bighead carp in China [J]. Chin. Sci. Bull., 1964, 10: 900-907.
7	钟麟.鲢鳙的池塘繁殖[J].科学通报, 1958, 21: 658-659.
	ZHONG L. Pond breeding of silver carp and bighead carp [J]. Chin. Sci. Bull., 1958, 21: 658-659.
8	朱宁生.青、鲩、鲢、鳙等家鱼催情试验的初步报告[J].水生生物学报, 1955(2): 60-69.
	ZHU N S. Preliminary experiments on induced ovulationof some economical cyprinids [J]. Acta Hydrobiol. Sin., 1955(2): 60-69.
9	徐康,段巍,肖军,等.鱼类遗传育种中生物学方法的应用及研究进展[J].中国科学: 生命科学, 2014, 44(12): 1272-1288.
	XU K, DUAN W, XIAO J, et al.. Development and application of biological technologies in fish genetic breeding [J]. Sci. Sin. Vitae, 2014, 44(12): 1272-1288.
10	刘少军.鱼类远缘杂交[M].北京:科学出版社, 2015:1-358.
	LIU S J. Fish Distance Hybridization [M]. Beijing: Science Press, 2015:1-358.
11	王石,汤陈宸,陶敏,等.鱼类远缘杂交育种技术的建立及应用[J].中国科学:生命科学, 2018, 48(12): 1310-1329.
	WANG S, TANG C C, TAO M, et al.. Establishment and application of distant hybridization technology in fish [J]. Sci. Sin. Vitae, 2018, 48(12): 1310-1329.
12	LIU S J, LUO J, CHAI J, et al.. Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross [J]. Proc. Natl. Acad. Sci. USA, 2016, 113(5): 1327-1332.
13	REN L, LI W, QIN Q, et al.. The subgenomes show asymmetric expression of alleles in hybrid lineages of Megalobrama amblycephala × Culter alburnus [J]. Genome Res., 2019, 29(11): 1805-1815.
14	桂建芳,肖武汉,梁绍昌,等.静水压休克诱导水晶彩鲫三倍体和四倍体的细胞学机理初探[J].水生生物学报, 1995, 19(1): 49-55.
	GUI J F, XIAO W H, LIANG S C, et al.. Preliminary study on the cytological mechanism of triploidy and tetraploidy induced by hydrostatic pressure shock in transparent colored crucian carp [J]. Acta Hydrobiol. Sin., 1995, 19(1): 49-55.
15	陈松林,李文龙,季相山,等.半滑舌鳎三倍体鱼苗的人工诱导与鉴定[J].水产学报, 2011, 35(6): 925-931.
	CHEN S L, LI W L, JI X S, et al.. Induction and identification of artificial triploid fry in Cynoglossus semilaevis [J]. J. Fish China, 2011, 35(6): 925-931.
16	蒋一珪,梁绍昌,陈本德,等.异源精子在银鲫雌核发育子代中的生物学效应[J].水生生物学报, 1983, 8(1): 1-13.
	JIANG Y G, LIANG S C, CHEN B D, et al.. Biological effect of heterologous sperm on gynogenetic offspring in Carassius auratus gibelio [J]. Acta Hydrobiol. Sin., 1983, 8(1): 1-13.
17	桂建芳,周莉.多倍体银鲫克隆多样性和双重生殖方式的遗传基础和育种应用[J].中国科学: 生命科学, 2010, 40(2): 97-103.
	GUI J F, ZHOU L. Genetic basis and breeding application on clonal diversity and dual reproduction modes in polyploid Carassius auratus gibelio [J]. Sci. Sin. Vitae, 2010, 40(2): 97-103.
18	WANG Y, LI X Y, XU W J, et al.. Comparative genome anatomy reveals evolutionary insights into a unique amphitriploid fish [J]. Nat. Ecol. Evol., 2022, 6:1354-1366.
19	张晓娟,周莉,桂建芳.遗传育种生物技术创新与水产养殖绿色发展[J].中国科学:生命科学, 2019, 49(1): 1409-1429.
	ZHANG X J, ZHOU L, GUI J F. Biotechnological innovation in genetic breeding and sustainable green development in Chinese aquaculture [J]. Sci. Sin. Vitae, 2019, 49(1):1409-1429.
20	陈戟,胡炜,朱作言.鱼类生殖发育调控研究进展[J].科学通报, 2013, 58(2): 103-114.
	CHEN J, HU W, ZHU Z Y. Progress in studies of fish reproductive development regulation [J]. Chin. Sci. Bull., 2013, 58(2): 103-114.
21	梅洁,桂建芳.鱼类性别异形和性别决定的遗传基础及其生物技术操控[J].中国科学:生命科学, 2014, 44(12): 1198-1212.
	MEI J, GUI J F. Genetic basis and biotechnological manipulation of sexual dimorphism and sex determination in fish [J]. Sci. Sin. Vitae, 2014, 44(12): 1198-1212.
22	CHEN J, ZHU Z, HU W. Progress in research on fish sex determining genes [J/OL]. Water Biol. Security, 2022, 1(1): 100008 [2022-09-22]. .
23	陶彬彬,胡炜.鱼类性别控制育种研究进展[J].中国农业科技导报,2022, 24(2): 1-10.
	TAO B B, HU W. Research progress on sex control breeding of fish [J]. J. Agric. Sci. Technol., 2022, 24(2): 1-10.
24	ZHU Z Y, LI G L, HE L, et al.. Novel gene transfer into the fertilized eggs of gold fish (Carassius auratus L. 1758) [J]. J. Appl. Ichthyol., 1985, 1:31-34.
25	朱作言,许克圣,谢岳峰,等.转基因鱼模型的建立[J].中国科学:B 辑,1989(2): 147-155.
	ZHU Z Y, XU K S, XIE Y F, et al.. Establishment of transgenic fish model [J]. Sci. Sin. Chim. B, 1989(2): 147-155.
26	叶鼎,朱作言,孙永华.鱼类基因组操作与定向育种[J].中国科学:生命科学, 2014, 44(12): 1253-1261.
	YE D, ZHU Z Y, SUN Y H, et al.. Fish genome manipulation and directional breeding [J]. Sci. Sin. Vitae, 2014, 44(12): 1253-1261.
27	胡炜,朱作言.美国转基因大西洋鲑产业化对我国的启示[J].中国工程科学, 2016, 18(3): 105-109.
	HU W, ZHU Z Y. Enlighenments for China from the industrialization of the transclenic Atlantic salmon in the US [J]. Strategic Study CAE, 2016, 18(3): 105-109.
28	PANG S C, WANG H P, ZHU Z Y, et al.. Transcriptional activity and DNA methylation dynamics of the Gal4/UAS system in zebrafish [J]. Mar. Biotechnol., 2015, 17(5): 593-603.
29	ZHANG X, PANG S, LIU C, et al.. A novel dietary source of EPA and DHA: metabolic engineering of an important freshwater species-common carp by fat1-transgenesis [J]. Mar. Biotechnol., 2019, 21(2): 171-185.
30	REES H A, LIU D R. Base editing: precision chemistry on the genome and transcriptome of living cells [J]. Nat. Rev. Genet., 2018, 19(12): 770-788.
31	ANZALONE A V, RANDOLPH P B, DAVIS J R, et al.. Search-and-replace genome editing without double-strand breaks or donor DNA [J]. Nature, 2019, 576(7785): 149-157.
32	SUN Y H, ZHANG B, LUO L F, et al.. Systematic genome editing of the genes on zebrafish chromosome 1 by CRISPR/Cas9 [J]. Genome Res., 2020, 30(1): 118-126.
33	DONG Z J, GE J C, LI K, et al.. Heritable targeted inactivation of Myostatin gene in yellow catfish (Pelteobagrus fulvidraco) using engineered zinc finger nucleases [J/OL]. PLoS One, 2011, 6(12): e28897 [2022-09-22]. .
34	YANO A, GUYOMARD R, NICOL B, et al.. An immune-related gene evolved into the master sex-determining gene in rainbow trout, Oncorhynchus mykiss [J]. Curr. Biol., 2012, 22(15): 1423-1428.
35	LI M H, YANG H H, LI M R, et al.. Antagonistic roles of Dmrt1 and Foxl2 in sex differentiation via estrogen production in tilapia as demonstrated by TALENs [J]. Endocrinology, 2013, 154(12): 4814-4825.
36	QIN Z K, LI Y, SU B F, et al.. Editing of the luteinizing hormone gene to sterilize channel catfish, Ictalurus punctatus, using a modified zinc finger nuclease technology with electroporation [J]. Mar. Biotechnol., 2016, 18(2): 255-263.
37	FENG K, LUO H R, LI Y M, et al.. High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases [J]. Sci. Bull., 2017, 62(3): 162-164.
38	ZHONG Z, NIU P, WANG M, et al.. Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp [J/OL]. Sci. Rep., 2016, 6: 22953 [2022-09-22]. .
39	CUI Z K, LIU Y, WANG W W, et al.. Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis) [J/OL]. Sci. Rep., 2017, 7: 42213 [2022-09-22]. .
40	LIU Q F, QI Y H, LIANG Q L, et al.. Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny [J]. Sci. China Life Sci., 2019, 62(9): 1194-1202.
41	CHEN J, WANG W, TIAN Z H, et al.. Efficient gene transfer and gene editing in sterlet (Acipenser ruthenus) [J/OL]. Front. Genet., 2018, 9:117 [2022-09-22]. .
42	TAO B, TAN J, CHEN L, et al.. CRISPR/Cas9 system-based myostatin-targeted disruption promotes somatic growth and adipogenesis in loach, Misgurnus anguillicaudatus [J/OL]. Aquaculture, 2021, 544: 737097 [2022-09-22]. .
43	NIE C H, WAN S M, CHEN Y L, et al.. Single-cell transcriptomes and runx2b ^-/- mutants reveal the genetic signatures of intermuscular bone formation in zebrafish [J/OL]. Natl. Sci. Rev., 2022, 9: nwac152 [2022-09-22]. .
44	XU H, TONG G, YAN T, et al.. Transcriptomic analysis provides insights to reveal the bmp6 function related to the development of intermuscular bones in zebrafish [J/OL]. Front. Cell Dev. Biol., 2022, 10: 821471 [2022-09-22]. .
45	STAR B, NEDERBRAGT A J, JENTOFT S, et al.. The genome sequence of Atlantic cod reveals a unique immune system [J]. Nature, 2011, 477(7363): 207-210.
46	CHEN S L, ZHANG G J, SHAO C W, et al.. Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle [J]. Nat. Genet., 2014, 46(3): 253-260.
47	BERTHELOT C, BRUNET F, CHALOPIN D, et al.. The rainbow trout genome provides novel insights into evolution after whole-genome duplication in vertebrates [J/OL]. Nat. Commun., 2014, 5: 3657 [2022-09-22]. .
48	WU C W, ZHANG D, KAN M Y, et al.. The draft genome of the large yellow croaker reveals well-developed innate immunity [J]. Nat. Commun., 2014, 5: 6227 [2022-09-22]. .
49	XU P, ZHANG X, WANG X, et al.. Genome sequence and genetic diversity of the common carp, Cyprinus carpio [J]. Nat. Genet., 2014, 46(11): 1212-1219.
50	WANG Y P, LU Y, ZHANG Y, et al.. The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation [J]. Nat. Genet., 2015, 47(8): 625-631.
51	SHAO C W, BAO B L, XIE Z Y, et al.. The genome and transcriptome of Japanese flounder provide insights into flatfish asymmetry [J]. Nat. Genet., 2017, 49(1): 119-124.
52	DU K, STOCK M, KNEITZ S, et al.. The sterlet sturgeon genome sequence and the mechanisms of segmental rediploidization [J]. Nat. Ecol. Evol., 2020, 4(6): 841-852.
53	HOUSTON R D, BEAN T P, MACQUEEN D J, et al.. Harnessing genomics to fast-track genetic improvement in aquaculture [J]. Nat. Rev. Genet., 2020, 21(7): 389-409.
54	石米娟,张婉婷,程莹寅,等.基于全基因组分析技术的鱼类育种技术原理与应用 [J]. 中国农业科技导报, 2022, 24(2): 33-41.
	SHI M J, ZHANG W T, CHENG Y Y, et al.. Fish breeding technology based on whole genome analysis and its application [J]. J. Agric. Sci. Technol., 2022, 24(2): 33-41.
55	宋海亮,胡红霞.基因组选择及其在水产动物育种中的研究进展[J].农业生物技术学报, 2022, 30(2): 379-392.
	SONG H L, HU H X. Genomic selection and its research progress in breeding of aquaculture species [J]. Chin. J. Agric. Biotechol., 2022, 30(2): 379-392.
56	CIRUNA B, WEIDINGER G, KNAUT H, et al.. Production of maternal-zygotic mutant zebrafish by germ-line replacement [J]. Proc. Natl. Acad. Sci. USA, 2002, 99(23): 14919-14924.
57	TAKEUCHI Y, YOSHIZAKI G, TAKEUCHI T. Surrogate broodstock produces salmonids [J]. Nature, 2004, 430(7000): 629-630.
58	JIN Y H, ROBLEDO D, HICKEY J M, et al.. Surrogate broodstock to enhance biotechnology research and applications in aquaculture [J]. Biotechnol. Adv., 2021, 49: 107756 [2022-09-22]. .
59	ZHANG F H, LI X M, HE M D, et al.. Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells [J]. J. Genet. Genomics, 2020, 47(1): 37-47.
60	ZHANG F H, HAO Y K, LI X M, et al.. Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation [J]. Sci. China Life Sci., 2022, 65(5): 969-987.
61	GUI J F, ZHOU L, LI X Y. Rethinking fish biology and biotechnologies in the challenge era for burgeoning genome resources and strengthening food security [J/OL]. Water Biol. Security, 2022, 1(1): 100002 [2022-09-22]. .
62	胡炜,汪亚平,朱作言.转基因鱼生态风险评价及其对策研究进展[J].中国科学: 生命科学, 2007, 37(4): 377-381.
	HU W, WANG Y P, ZHU Z Y. Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies [J]. Sci. Sin. Vitae, 2007, 37(4): 377-381.
63	ZHANG Y, CHEN J, CUI X, et al.. A controllable on-off strategy for the reproductive containment of fish [J/OL]. Sci. Rep., 2015, 5: 7614 [2022-09-22]. .
64	FAN G Y, SONG Y, YANG L D, et al.. Initial data release and announcement of the 10,000 Fish Genomes Project (Fish10K) [J/OL]. Gigascience, 2020, 9(8): giaa080 [2022-09-22]. .
65	OKUTSU T, SHIKINA S, SAKAMOTO T, et al.. Successful production of functional Y eggs derived from spermatogonia transplanted into female recipients and subsequent production of YY supermales in rainbow trout, Oncorhynchus mykiss [J]. Aquaculture, 2015, 446: 298-302.
66	WANG Y Q, YE D, ZHANG F H, et al.. Cyp 11a2 is essential for oocyte development and spermatogonial stem cell differentiation in zebrafish [J/OL]. Endocrinology, 2022, 163(2):bqab258 [2022-09-22]. .
67	LI X M, ZHANG F H, WU N, et al.. A critical role of foxp3a-positive regulatory T cells in maintaining immune homeostasis in zebrafish testis development [J]. J. Genet. Genomics, 2020, 47(9): 547-561.

[1]	阿布都克尤木·阿不都热孜克, 古丽米拉·艾克拜尔, 徐麟, 颜国荣, 刘宁, 赵连佳, 邓超宏, 帕丽旦·艾海提, 王威. 我国农业植物新品种保护发展回顾、现状分析及发展建议[J]. 中国农业科技导报, 2022, 24(9): 1-11.
[2]	王帅, 宋伟, 王荣焕, 赵久然. 我国玉米生物学研究进展[J]. 中国农业科技导报, 2022, 24(7): 23-31.
[3]	崔遵康, 李丹阳, 徐小婷, 朱俊峰, 武拉平, 左文革. 粮食作物生物育种技术全球创新布局与竞争态势研究——基于核心专利数据挖掘的视角[J]. 中国农业科技导报, 2022, 24(5): 1-14.
[4]	易媛, 张会云, 刘立伟, 王静, 朱雪成, 赵娜, 冯国华. 活性腐殖酸缓释肥替代尿素对徐麦新品种产量和群体质量的影响[J]. 中国农业科技导报, 2022, 24(4): 144-153.
[5]	陶彬彬, 胡炜. 鱼类性别控制育种研究进展[J]. 中国农业科技导报, 2022, 24(2): 1-10.
[6]	常亚青\|田燚\|张伟杰. 我国海洋水产生物遗传育种技术进展[J]. , 2013, 15(6): 8-15.
[7]	王立平1,刘平1,吕波1,张新明1,周建仁2. 我国农林植物新品种保护现状与展望[J]. , 2010, 12(6): 62-67.
[8]	张守攻1,齐力旺1,尹刚强2. 速生高抗林木新品种高效培育技术体系与产业化[J]. , 2010, 12(3): 1-7.
[9]	耿晓君1,周波2. 水稻新品种异地生态鉴定试验丰产性、稳产性研究[J]. , 2009, 11(S2): 37-42.
[10]	易代勇,周正邦,朱文华,李向勇,王代谷. 贵引07-15等果蔗新品种(系)高产、稳产及适应性的分析评价[J]. , 2009, 11(S2): 74-77.
[11]	景东林. 高产、稳产小麦新品种——邢麦6号[J]. , 2009, 11(S2): 110-111.
[12]	杜运科,任雅琴,陈三乐,孙军仓,王忠景. 国审小麦新品种秦农142选育研究[J]. , 2009, 11(S2): 112-115.
[13]	柳迅生,谢居林,杨天英,覃志顺,王涛,王永华,张洪龙,丁丽. 优质高产大豆新品种安豆5号的选育与应用[J]. , 2009, 11(S2): 130-132.
[14]	聂智星,杭晓宁,罗佳,王苗,王云龙,吴小园,赵团结. 以农民为中心的参与式植物育种研究进展[J]. , 2008, 10(6): 48-55.
[15]	崔艳,郭德忠. 农业转基因植物的法律监管与保护[J]. , 2008, 10(2): 51-55.