%0 Journal Article %A CONG Xiao-fei %A DING Jun* %A CHANG Ya-qing %T Development and Polymorphism Analysis of SNP |Marker in LYZ of Strongylocentrotus intermedius %D 2012 %R 10.3969/j.issn.1008-0864.2012.05.21 %J Journal of Agricultural Science and Technology %P 136-144 %V 14 %N 5 %X

Mutation scanning of Strongylocentrotus intermedius genes was conducted by high-resolution melting curve (HRM) analysis technology. 26 alternative SNP loci were screened out. Among them, 16 were base substitution accounting for 61.54%, 6 were base deletion or insertion accounting for 23.08%. The number of missing bases was ranging from 1 to 4. 4 are fuzzy base substitution type and tri-state loci accounting for 15.38%. According to these figures, SNP frequency distribution in this gene is about 2.85%, and 18 loci in the coding region, among which 2 are missense mutations. We analyzed 5 SNP loci using small amplicon genotyping in 60 individuals from 2 populations from different regions. The results indicated that all 5 loci had 2 alleles, the minor allele frequency ranged from 0.150 0 to 0.500 0, and SILyz-7, SILyz-15 and SILyz-20 had large differences in minor allele frequency between 2 populations. It could be speculated that HRM is an efficient and reliable SNP analysis method, and the BNP marker could be used for studying Strongylocentrotus intermedius genetics and breeding.

%U https://www.nkdb.net/EN/10.3969/j.issn.1008-0864.2012.05.21