%0 Journal Article %A HAN Jian-bo %A LI Qiu-ling %A WANG Chang-fa %A et al. %T A New SNP in Coding Region of hsp70 Gene and the Association of  Polymorphism with Heat Stress Traits in Chinese Holstein Cattle %D 2009 %R %J Journal of Agricultural Science and Technology %P 56-63 %V 11 %N 3 %X

In order to explore the association of hsp70 gene polymorphism with heat stress traits \[rectal temperature (RT) and potassium content in erythrocytes (PCE)\] in 290 heads of Chinese Holstein cattle, Polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) was used to detect possible single nucleotide polymorphisms (SNPs) in hsp70 gene, genotypes were analyzed by the PCR-restriction fragment length polymorphism (RFLP) technique, SBYE method was used to quantify hsp70 mRNA expression in different tissues through fluorescent quantitative reverse transcription (RT)-PCR assay. We found a G/A SNP at nucleotide 1524, resulting two genotypes of AA and AB. Allele and genotype frequencies were 0.957 0 and 0.043 0, and 0.913 7 and 0.086 3, respectively. χ2 test showed that the population was at Hardy-Weinberg equilibrium. Association analysis indicated that there was no difference in RT between AA and AB genotypes, however, the PCE was significantly different between AA and AB genotypes. The results of the fluorescent quantitative RTPCR study showed that the expression of hsp70 mRNA in the liver was significantly higher than other tissues (P<0.05). We presume that the G/A SNP at nucleotide 1524 in the hsp70 gene might be in association with heat stress in Chinese Holstein cattle.

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