%0 Journal Article %T Single Nucleotide Polymorphism in the Promoter Region of MBL1 Gene in Bos Taurus %D 2011 %R 10.3969/j.issn.1008-0864.2011.02.06 %J Journal of Agricultural Science and Technology %P 38-45 %V 13 %N 2 %X

In order to study the single nucleotide polymorphisms (SNPs) in the promoter region of Mannose-binding Lectin1, the genotypes of 3 loci at -2194(A>C),-1446(T>C),-1330(G>A) in the promoter region of MBL1 were detected by RFLP (restriction fragment length polymorphism), SSCP (single-strand conformation polymorphism) and directly sequencing method, with 1 073 Chinese Holstein cattles, 69 Luxi Yellow cattle and 24 Bohai Black cattles as materials. The results showed that only 2 genotypes (AA/AC,GG/GA) were observed at sites -2194(A>C)and -1330(G>A)in Bohai Black cattle, and in Luxi Yellow cattles, the genotype AA was not detected at site -1330 (G>A). The dominant alleles in 3 breeds were A, C and G, and the allelic frequencies were A (CH 0.900 1/LYC 0.760 9/BBC 0.937 5)、C (CH 0.604 8/LYC 0.768 1/BBC 0.625) and G(CH 0.725 1/LYC 0.869 6/BBC 0.895 8),  respectively. The above results indicated that among all detected loci, the dominant alleles in 3 breeds were conservative relatively. Thus the 3 SNPs association with production traits needs   further studies.

%U https://www.nkdb.net/EN/10.3969/j.issn.1008-0864.2011.02.06