Abstract: The seeds of Notopterygium incisum, a traditional Chinese medicine plant, are dormant, which limits its artificial domestication and cultivation. In order to analyze the metabolite changes and screen the key compounds during releasing dormancy of N. incisum seeds, seeds of N. incisum were pretreated with cold and warm stratification; and the pretreated seeds were treated with low temperature stratification (CS), ABA inhibitor fluridone (FL) and GA₃ (GA), respectively. Then seeds of different treatments were detected with UPLC-MS/MS. The results showed that the whole treated process of N. incisum seeds could be divided into three stages: 0—60 d, 90—(150+30) d and (150+60)—(150+110) d, among which N. incisum seeds treated with  pretreatment+CS for (150+60) and (150+110) d  had germination capablity. The compounds represented by the principal component t[1] were the compounds that had undergone major changes of seed embryos during 0, 15, 60, 90, 120 d after pretreatments. The compounds represented by the principal component of t[2] were mainly changed during the release of physiological dormancy. The difference analysis showed that the content of orobanchyl acetate, a strigolactone compound, was accumulated significantly in released dormancy of N. incisum seed, which was increased by 12.1 times after FL treatment and 28.5 times after CS treatment. Therefore, orobanchyl acetate could be  crucial during the dormancy release of N. incisum seeds. This study provided a theoretical basis for dormancy release mechanism of N. incisum seeds and a practical significance for the development of Chinese medicine industry of N. incisum.

Key words: Notopterygium incisum, seed dormancy, metabolotics, strigolactones, orobanchyl acetate

摘要： 传统中药植物羌活的种子因具有休眠限制了其人工驯化和栽培种植。为了解析羌活种子休眠解除过程的代谢物变化，筛选休眠解除关键化合物，对羌活种子进行冷暖层积预处理150 d，并对预处理的种子进行低温层积处理110 d，以及ABA抑制剂氯定酮（FL）和GA₃（GA）处理，用UPLC-MS/MS对处理过程的羌活种子进行代谢物分析。结果表明：羌活种子整个处理过程分为3个阶段，0—60 d、90—（150+30） d、（150+60）—（150+110） d。其中，预处理+低温层积处理（150+60） d和（150+110） d 的羌活种子已具备萌发能力。t[1] 主成分所代表的化合物是预处理0、15、60、90、120 d的胚发育处理过程发生主要变化的化合物。t[2] 主成分所代表的化合物主要在生理休眠解除过程发生变化。差异分析发现，列当醇乙酸酯是一种独脚金内酯化合物，在种子休眠解除过程发生显著积累。其中，FL处理后列当醇乙酸酯的生物合成水平升高12.1倍，而低温层积处理后其合成水平升高28.5倍。因此，推测列当醇乙酸酯含量变化对羌活种子休眠解除非常关键。研究结果可为羌活种子休眠解除机理研究提供理论基础，对中药羌活的产业发展具有重要的实践意义。

关键词: 羌活, 种子休眠, 代谢组, 独脚金内脂, 列当醇乙酸酯