关键词: 卵-胚胎;输卵管;子宫;卵母细胞;卵丘细胞

Abstract:

In order to clarify the fertilisation and early development or the progression of unfertilised eggs in the oviduct of domestic farm animals, four transplant studies are described in the study. ① Pig eggs transplanted from ovulations induced during the luteal phase of the oestrous cycle were fertilised in the oviducts of inseminated oestrous animals. By contrast, pig eggs from oestrous donors became highly polyspermic when transplanted to the oviducts of animals forcemated during the luteal phase. ② Pig embryos at the stage of hatched blastocysts (7 d and 8 d) could be transplanted successfully to synchronous recipients and full embryonic development be demonstrated between 19 d and 23 d. Thus, the exposed trophectoderm of enlarging embryos could withstand the physical manipulation of recovery and transplantation, and the lifespan of corpora lutea in the unmated recipients could be prolonged after 7 d and 8 d transfered. ③ Bovine oocytes aspirated from 2~6 mm diameter Graafian follicles and matured in vitro were fertilised normally in the oviducts of inseminated recipient heifers, demonstrating the potential of slaughterhouse ovaries for the generation of embryos. ④ Transplanting equine eggs to a pig oviduct, in which egg descent to the uterus requires only 46~48 h, did not reveal a retarded progress of degenerating unfertilised horse eggs, suggesting the involvement of nonphysical factors in equine embryo progression to the uterus. Prostaglandins of embryonic origin are now known to be a key. A final section examines the postovulatory rle of ovarian follicular cells on the secretory activity of the oviduct epithelium. Based on above results, egg transfer is an effective vintage analytical tool in domestic animals.

Key words: Eggembryo, oviduct, uterus, oocyte, cumulus cell