基于SRAP的西瓜种质资源遗传多样性评价

doi:doi:10.3969/j.issn.1008-0864.2013.02.14

›› 2013, Vol. 15 ›› Issue (2): 89-96.DOI: doi:10.3969/j.issn.1008-0864.2013.02.14

基于SRAP的西瓜种质资源遗传多样性评价

李朋飞,霍秀爱,程永强,代亮,杨炳艳,段会军*

(河北农业大学河北省作物种质资源实验室, 河北保定 071001)

收稿日期:2012-11-26 修回日期:2013-01-10 出版日期:2013-04-15 发布日期:2013-04-16
通讯作者: 段会军，教授，博士，研究方向为西瓜遗传育种。E-mail:huijunduan2000@yahoo.com.cn
作者简介:李朋飞|硕士研究生|研究方向为西瓜遗传育种。E-mail:lipengfei0218@126.com。
基金资助:
河北省自然科学基金项目(C2007000547)资助。

Assessment of Genetic Diversity in Watermelon Based on SRAP Analysis

LI Peng-fei, HUO Xiu-ai, CHENG Yong-qiang, DAI Liang, YANG Bing-yan, DUAN Hui-jun*

(Crop Germplasm Resources Laboratory of Hebei Province, Agricultural University of Hebei, Hebei Baoding 071001, China)

Received:2012-11-26 Revised:2013-01-10 Online:2013-04-15 Published:2013-04-16

摘要/Abstract

摘要：

运用SRAP分子标记技术分析了80份西瓜种质资源的遗传多样性，28对不同的引物组合共扩增出308条谱带，其中多态性谱带68条。引物组合的多态性频率最低为9.1%，最高为45.5%，平均22.1%。基于全部材料的SRAP基因型数据评价品种间的遗传关系，80份西瓜种质的遗传相似系数变化范围在0.94～1.00之间，表明供试西瓜种质资源的遗传背景比较狭窄。利用NTSYS软件聚类分析，80份西瓜材料被划分为3个类群，类群间相似度较高。来源地不同的西瓜材料也被划分到一个类群，进一步说明供试材料遗传背景狭窄。西瓜育种要取得突破性进展，必须加强种质资源的引进与创新。

关键词: 西瓜;SRAP;遗传多样性

Abstract:

Eighty watermelon materials were analyzed by SRAP (sequence-related amplified porlymorphism) technique. The results showed that 308 bands were amplified using 28 primer pairs, and 68 bands were polymorphic. Among the primer pairs, the minimum polymorphism frequency is 9.1%, the maximum is 45.5%, and the average is 22.1%. Based on DNA bands, the similarity coefficients were calculated and their value were ranged from 0.94～1.00, which indicated that their genetic base was narrow. Clustering analysis revealed that 80 watermelon materials were classified into 3 groups. The values of similarity coefficient among them were high. Materials from different districts were clustered together, which also showed that their genetic background was narrow. In order to get breakthrough and progress in watermelon breeding, we must strengthen the introduction and innovation of watermelon germplasm resources.

Key words: watermelon, SRAP, genetic diversity

中图分类号:

S651.01

李朋飞,霍秀爱,程永强,代亮,杨炳艳,段会军*. 基于SRAP的西瓜种质资源遗传多样性评价[J]. , 2013, 15(2): 89-96.

LI Peng-fei, HUO Xiu-ai, CHENG Yong-qiang, DAI Liang, YANG Bing-yan, DUAN Hui-ju. Assessment of Genetic Diversity in Watermelon Based on SRAP Analysis[J]. , 2013, 15(2): 89-96.

基于SRAP的西瓜种质资源遗传多样性评价

Assessment of Genetic Diversity in Watermelon Based on SRAP Analysis

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