关键词: 非酶连;PCR;基因组步移;染色体步移

Abstract:

PCR-mediated approaches for genomic walking (or chromosome walking) are routinely used for cloning flanking sequences of the known DNA regions, which is an important technology for genomic research. According to the principles, they can be categorized into two types of ligation-dependence and ligation-independence. At present, numerous approaches of the later, each with distinct features, have been rapidly developed. Nevertheless, regarding the binding patterns of walking primers on DNA templates, they can be overall grouped into the degenerate primer-based or specific sequence-dependent subsets. Following this viewpoint, this paper summarized the major or representative techniques and the recent developments of ligation-independent, PCR-mediated genomic walking, so as to provide a methodological reference for the relevant researches.

Key words: ligation-independence, PCR, genomic walking, chromosome walking