巴氏芽孢八叠球菌诱变选育脲酶高产菌株

doi:doi:10.3969/j.issn.10080864.2013.06.19

›› 2013, Vol. 15 ›› Issue (6): 130-134.DOI: doi:10.3969/j.issn.10080864.2013.06.19

巴氏芽孢八叠球菌诱变选育脲酶高产菌株

李萌1,2,程晓辉2*,杨钻2,郭红仙2

(1.北京工商大学理学院, 北京市植物资源研究开发重点实验室, 北京 100048|2.清华大学土木工程系, 北京 100084)

出版日期:2013-12-15 发布日期:2013-12-16
通讯作者: 程晓辉，副教授，主要从事微生物加固与防渗的土建工程技术研究。Email:chengxh@tsinghua.edu.cn
作者简介:李萌|讲师|博士|主要从事微生物代谢产物研究。Email：limeng@th.btbu.edu.cn。
基金资助:
国家自然科学基金项目（50908122）资助。

Breeding High\|yield Urease\|producing Sporosarcina pasteurii Strain by NTG Mutation

LI Meng1,2, CHENG Xiaohui2*, YANG Zuan2, GUO Hongxian2

（1.Beijing Key Laboratory of Plant Resources Research and Development, School of Sciences, Beijing Technology and Business University, Beijing 100048|2.Department of Civil Engineering, Tsinghua University, Beijing 100084, China）

Online:2013-12-15 Published:2013-12-16

摘要/Abstract

摘要：

脲酶催化尿素的水解生成形成铵根离子和碳酸根离子，具有广泛的应用价值。以巴氏芽孢八叠球菌ATCC11859为出发菌株, 通过亚硝基胍诱变，得到高产脲酶突变株。其中菌株B\|20的脲酶活性最高，为28.4 mmol/min，较出发菌株提高了207%。对该菌株接种量、氮源种类培养条件进行优化，结果表明接种量为10%，且采用大豆粉为氮源时，脲酶活性最高为32.9 mmol/min。对脲酶高产菌株的选育表明该方法是一种行之有效的方法。

关键词: 巴氏芽孢八叠球菌；脲酶；亚硝基胍；诱变选育

Abstract:

Urease catalyzed hydrolysis of urea formed ammonium ions and carbonate ions. It has extensive application value. The strain of S. pasteurii ATCC11859 was screened through NTG (N\|methyl\|N″\|nitroso\|N\|nitrosoguanidine) mutation and a high yielding urease mutant was obtained. Among which strain B\|20 gave the highest urease activity of 28.37 mmol/min. This was increased 207% of the original strain. The strain inoculation amount and culture conditions of nitrogen source type were optimized. The results showed that the inoculation quantity was 10%, and using soybean meal as nitrogen source could achieve maximum urease activity as high as 32.9 mmol/min. This indicated that the technology is effective method for breeding high\|yield urease strain.

Key words: Sporosarcina pasteurii, urease, nitrosoguanidine(NTG), mutation breeding

中图分类号:

李萌1,2,程晓辉2*,杨钻2,郭红仙2. 巴氏芽孢八叠球菌诱变选育脲酶高产菌株[J]. , 2013, 15(6): 130-134.

LI Meng1,2, CHENG Xiaohui2*, YANG Zuan2, GUO Hongxian2. Breeding High\|yield Urease\|producing Sporosarcina pasteurii Strain by NTG Mutation[J]. , 2013, 15(6): 130-134.

[1]	陈洪1,赵元弟2,魏芳1,董绪燕1,马志亚2. 新型纳米脂质功能食品研究进展[J]. , 2011, 13(5): 64-72.
[2]	王喆. 我国食品工业的科技发展对策[J]. , 2006, 8(5): 61-65.

巴氏芽孢八叠球菌诱变选育脲酶高产菌株

Breeding High\|yield Urease\|producing Sporosarcina pasteurii Strain by NTG Mutation

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics