关键词: 细菌脂肪酶;脂肪酶特异折叠酶;立体分子伴侣;蛋白折叠

Abstract:

Lipases represent the most important class of enzymes used in industry. The enzymes originating from Pseudomonas and Burkholderia species are wildly used. But a huge energetic barrier traps kinetically disable lipases in folding state to fold rightly. Research found that these enzymes were usually encoded in an operon together with a second gene, which coded for a lipase-specific-foldase, Lif. It is necessary for obtaining enzymatically active lipase. Lifs are so-called steric chaperones, which exert their physiological function by lowering energetic barriers when folding their cognate lipases, thereby providing essential steric information needed for folding lipases into their enzymatically active conformation. In this review, we elaborated the discovery, classification, mechanism, and perspective for application.

Key words: bacteria lipase, lipase specific foldase, steric chaperone, protein folding