关键词: 蜂胶；提取率；总黄酮含量；响应曲面

Abstract:

Propolis has always been utilized as functional food for its superior health effect. In order to improve the quality and production efficiency of propolis products, on the basis of fully simulating the actual production conditions, we analyzed the influencing factors (extracting time, ethanol concentration, solid-liquid ratio, distilling frequency and distilled temperature) to extraction ratio and general flavonoids content of propolis by response surface methodology (RSM). The results showed that extraction ratio and general flavoniods content predicted by regression model for the propolis samples were 38.324 6% and 199.776 mg/g, respectively in this study. The optimal ethanolic extraction conditions of propolis were as fallowing: extracting time was 24 h, ethanol concentration was 95%, solid-liquid ratio was 10∶1, extracting frequency was twice, and extracting temperature was room temperature. In order to reach the objective of guiding the production, this experiment conclusion should be inspected by the actual production workshop.

Key words: propolis, extraction ratio, general flavoniods content, response surface methodology