脱氧核糖核酸定量的潜在基准方法研究进展

doi:10.3969/j.issn.10080864.2013.01.25

›› 2013, Vol. 15 ›› Issue (1): 158-163.DOI: 10.3969/j.issn.10080864.2013.01.25

脱氧核糖核酸定量的潜在基准方法研究进展

李亮1,张秀杰1,宛煜嵩1,楚燕春2,金芜军1*

(1.中国农业科学院生物技术研究所, 农业部转基因植物用微生物环境安全监督检验测试中心(北京), 北京 100081|2.农业部科技发展中心, 北京 100122)

出版日期:2013-02-15 发布日期:2013-02-26
通讯作者: 金芜军,副研究员,博士,主要从事转基因生物安全评价与检测研究。Email:jinwujun1218@yahoo.com.cn
作者简介:李亮,助理研究员,博士,主要从事分子生物学与生物计量研究。Email:liliangene@yahoo.com.cn。
基金资助:
国家转基因生物新品种培育重大专项(2011ZX08012003)资助。

Progress in Potential Primary Methods for the Quantification of Deoxyribonucleic Acid

LI Liang1, ZHANG Xiujie1, WAN Yusong1, CHU Yanchun2, JIN Wujun1*

(1.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences|Inspection and Testing Center for Environmental Risk Assessment Genetically Modified Plantrelated Microoganism (Beijing), Ministry of Agriculture, Beijing 100081|2.Development Center for Science &|Technology, Ministry of Agriculture, Beijing 100122, China)

Online:2013-02-15 Published:2013-02-26

摘要/Abstract

摘要：

脱氧核糖核酸定量分析是食品安全检验、医疗诊断、转基因检测与病原微生物鉴定等相关领域的基础。常见的定量方法种类繁多,如紫外分光光度法、荧光染料法和实时荧光定量PCR法等等,各有所长,但测量结果缺乏可靠性、可比性和一致性。随着研究的深入与应用要求的提高,基准方法成为了核酸分析以及核酸测量能力评估的关键。本文综述了近年来三种成为潜在脱氧核糖核酸定量基准方法的研究发展,评述了各种方法的技术优势、不足与应用潜力,以期为其相关研究和利用提供参考。

关键词: 脱氧核糖核酸定量;基准方法;IDMS;ICPOES/MS;dPCR

Abstract:

Quantification of deoxyribonucleic acid are key applications encompassing food safety testing, medical diagnostics, detection of GMOs and pathogen identification. Its broad applications have encouraged a rapid and sustained development of the technology, especially the primary methods, resulted in reliability, comparability, and consistency. The analysis of deoxyribonucleic acid and the testing capability are crucially dependent on the measurements by primary methods. In this review, we discuss the advances in development of primary methods, including the advantages and disadvantages. The prospects for the development and applications of primary methods are also discussed.

Key words: quantification of deoxyribonucleic acid, primary methods, IDMS, ICPMS/OES, dPCR

中图分类号:

李亮1,张秀杰1,宛煜嵩1,楚燕春2,金芜军1*. 脱氧核糖核酸定量的潜在基准方法研究进展[J]. , 2013, 15(1): 158-163.

LI Liang1, ZHANG Xiujie1, WAN Yusong1, CHU Yanchun2, JIN Wujun1*. Progress in Potential Primary Methods for the Quantification of Deoxyribonucleic Acid[J]. , 2013, 15(1): 158-163.

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脱氧核糖核酸定量的潜在基准方法研究进展

Progress in Potential Primary Methods for the Quantification of Deoxyribonucleic Acid

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