微芯片电泳与聚丙烯酰胺凝胶电泳的比较分析

doi:doi:10.3969/j.issn.1008-0864.2013.02.27

›› 2013, Vol. 15 ›› Issue (2): 173-178.DOI: doi:10.3969/j.issn.1008-0864.2013.02.27

微芯片电泳与聚丙烯酰胺凝胶电泳的比较分析

张莎莎1,郭新梅2,赵美爱1,裴玉贺2,宋希云2*

(1.青岛农业大学生命科学学院, 青岛市主要农作物种质资源创新与应用重点实验室, 山东青岛 266109|2.青岛农业大学农学与植物保护学院, 山东青岛 266109)

收稿日期:2012-11-06 修回日期:2012-11-28 出版日期:2013-04-15 发布日期:2013-04-16
通讯作者: 宋希云，教授，博士，研究方向为玉米遗传育种、生物化学与分子生物学。E-mail：songxy@qau.edu.cn
作者简介:张莎莎|硕士研究生|研究方向为玉米遗传育种。E-mail：yachun_1987@163.com。
基金资助:
“十二五”国家科技支撑计划项目（2011BAD35B01）;山东省农业良种工程；山东省泰山学者岗位（作物栽培与育种学）;青岛市科技计划项目（12-1-3-15-nsh和12-1-4-5-(11)-jch）;山东省玉米育种与栽培技术重点实验室开放课题项目；山东省现代农业产业技术体系创新团队项目（621071）资助。

Comparative Analysis on Microchip Electrophoresis Technology and Polyacrylamide Gel Electrophoresis

ZHANG Sha-sha1, GUO Xin-mei2, ZHAO Mei-ai1, PEI Yu-he2, SONG Xi-yun2*

(1.Qingdao Key Lab of Germplasm Innovation and Application of Major Crops, College of Life Science, Qingdao Agricultural University, Shandong Qingdao 266109|2.College of Agronomy and Plant Protection, Qingdao Agricultural University, Shandong Qingdao 266109, China)

Received:2012-11-06 Revised:2012-11-28 Online:2013-04-15 Published:2013-04-16

摘要/Abstract

摘要：

聚丙烯酰胺凝胶电泳是目前实验室常用的分离寡核苷酸的电泳技术，微芯片电泳是近几年新兴起的DNA/RNA快速分析系统。对玉米自交系8112、黑糯等SSR分子标记的PCR扩增结果进行两种方法的比较，旨在分析检验微芯片电泳在重复性和分辨率上的可靠性。与聚丙烯酰胺凝胶电泳相比，微芯片电泳无毒性、操作更简单、灵敏度和准确性高，而且重复性良好，今后在科研工作中将拥有更加广阔的前景。

关键词: 微芯片电泳;聚丙烯酰胺凝胶电泳;重复性;准确性

Abstract:

Polyacrylamide gel electrophoresis (PAGE) is usually applied in separation of oligonucleotides. Microchip electrophoresis is a new electrophoretic technology to detect the molecular size of DNA and RNA samples. In order to identify the repeatability and precision of microchip electrophoresis, PCR products of Black Glutinous Maize and self intersect line 8112 etc. were tested using PAGE and microchip electrophoresis, respectively. The results showed that compared with PAGE, the latter had no toxin, and could be operated easily, with high sensitivity and accuracy, and had better repeatability. So it might present broader prospects in scientific research in future.

Key words: microchip electrophoresis, polyacrylamide gel electrophoresis, repeatabilrty, accuracy

中图分类号:

Q503

张莎莎1,郭新梅2,赵美爱1,裴玉贺2,宋希云2*. 微芯片电泳与聚丙烯酰胺凝胶电泳的比较分析[J]. , 2013, 15(2): 173-178.

ZHANG Sha-sha1, GUO Xin-mei2, ZHAO Mei-ai1, PEI Yu-he2, SONG Xi-yun2*. Comparative Analysis on Microchip Electrophoresis Technology and Polyacrylamide Gel Electrophoresis[J]. , 2013, 15(2): 173-178.

微芯片电泳与聚丙烯酰胺凝胶电泳的比较分析

Comparative Analysis on Microchip Electrophoresis Technology and Polyacrylamide Gel Electrophoresis

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