关键词: 猪种, 肠道菌群, 454 焦磷酸测序, 16S rDNA

Abstract:

Pyrosequencing of 16S rDNA genes was used to analyze and compare the differences of intestinal bacteria communities of Bama miniature pigs (BAMA), Black Xiang pigs (BLACK) and Yorkshire-Landrace-Duroc pigs (CROSS). The results showed that the diversity and abundance of intestinal bacteria varied among BAMA,BLACK and CROSS. Weeksella, Comamonas and Mitsuokella genus containing pathogenic bacteria were only detected in BAMA intestines, but they were not detected in BLACK and CROSS intestines. The diversity and abundance of Fibrobacter and Ruminococcus genus containing cellulose decomposing bacteria were higher in BAMA intestines than those in BLACK and CROSS intestines. The high content of pathogens and cellulose degrading bacteria in the intestines of BAMA suggested the better tolerance of BAMA to harsh conditions and high resistance against disease. BAMA was equipped for high roughage-resistance and liable to feeding management.

Key words: pig breeds, intestinal bacteria community, 454 pyrosequencing, 16S rDNA