关键词: 咔唑, 生物降解, Sphingosinicella sp., 环境因素

Abstract: Carbazole-degrading bacterial strain B1 was isolated from an oil well sludge outlet in Qinghai Province. Degradation strain was screened by enriched culture, and analyzed, identified by physiological properties and 16S rDNA sequence, and the carbazole concentration of the culture was detected by HPLC. The degrading capacities of the strain under different pH, salt concentration and temperature, and the effect of adding carbon, nitrogen sources, and substrate concentration on degradation efficiency were discussed. Through identification, strain B1 was belonged to Sphingosinicella sp.. The 100 mg/L carbazole degradation rate of B1 in 72 h under optimum conditions 30℃ and 7.0 pH could reach 98%, while its degradation rate could be higher when salt concentration was less than 10 g/L. Besides, the degradation rate was significantly improved by adding 0.1 g/L glucose and ammonium sulfate, and the strain B1 could tolerate 700 mg/L carbazole concentration. The results indicated that strain B1 had highly-efficient carbazole degradation ability and favorable environmental adaptability.

Key words: carbazole, biodegradation, Sphingosinicella sp., environmental factor