关键词: 紫小麦, 花色苷, 高效提取, 抗氧化活性, 肿瘤抑制

Abstract: In order to provide an efficient method for extracting anthocyanins from purple wheat bran and understand its antioxidant and inhibitory effect on tumor cells, this experiment took the bran of purple wheat ‘Nongda 3753’ as material, extracted anthocyanin by the improved CTAB method and response surface methodology to optimize the extraction process. DMPD+ method was also used to measure the anthocyaninson antioxidant activity. HGC-27 and MGC-803 were used to determine the effect of anthocyaninson on tumor cells proliferation and invasion. The results indicated that the best process parameters of extraction were solid-liquid ratio 1 g∶3.2 mL, extraction time 1.1 h, extration number 3 times; and the yield was 3.40 g/kg. Antioxidant experiments showed that the antioxidant activity of anthocyanins was higher than that of water soluble vitamin E. Moreover, anthocyanins could inhibit the invasion and proliferation of gastric cancer cells HGC-27 and MGC-803. These results indicated that the improved CTAB extrcation method was easy to operate, and the anthocyanins extracted had higher purity and higher antioxidant activity and tumor inhibition effect.

Key words: purple wheat, anthocyanin, efficient extraction, antioxidant activity, tumor inhibition