关键词: 蚕豆, 内生菌, 固氮菌, 促生长特性

Abstract: In order to study plant growth-promoting activities of endophytic nitrogen-fixing bacteria in faba bean tissues, the related bacteria were isolated from leaves and roots of faba beans’ seedling and analyzed the relationship between the ability of dissolving phosphate and potassium and the ability of nitrogen fixation, cellulase production and pectinase production of these bacteria to identify potential strain that promote plant growth through.  8 strains were isolated from the seedling, in which 7 strains could dissolve inorganic phosphorus, 2 strains could dissolve potassium, 4 strains could product cellulose and 2 strains could product pectase. Sl24-3 from leaves had the highest activity of nitrogenase, and the secretory ability of cellulase and pectinase; SR23-1 had the ability of dissolving inorganic phosphorus and potassium. These results showed that nitrogen fixing bacteria in Vicia faba seedlings obviously promoted the growth, among which SL24-3 and SR23-1 were potential dominant growth promoting bacteria. This study  excavated the potential excellent growth promoting bacteria, which provided the theoretical basis for the in-depth study of endophyte promoting plant growth, and had practical value and significance.

Key words: faba bean (Vicia faba L.), endophyte, nitrogen-fixing bacteria, growth-promoting activities