关键词: 异养小球藻, 絮凝剂, 采收率, 响应面分析

Abstract: The small volume of heterotrophic Chlorella and the complex components of fermentation broth make it difficult in algae cell harvesting and high recovery cost. In this study, five flocculant  of ferric chloride, aluminum sulfate, chitosan, polyacrylamide and mold  were compared, single factor test and response surface analysis were used to optimize the process conditions of collecting heterotrophic Chlorella by ferric chloride flocculation method, and a quadratic regression model was obtained. The optimal variables were the dosage of ferric chloride 2.0 g·L^-1, agitation rate 150 r·min^-1, agitation time 20 min. Under this condition, the recovery rate reached 95.3%, and the collection cost was 0.07 yuan·kg^-1. The recovery rate was high and the recovery cost was low, which laid the foundation for the industrialization application.

Key words: heterotrophic Chlorella, flocculant, recovery rate, response surface analysis