大豆鲜荚籽粒上位性QTL及其互作效应分析

doi:10.13304/j.nykjdb.2020.1057

中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (8): 25-36.DOI: 10.13304/j.nykjdb.2020.1057

大豆鲜荚籽粒上位性QTL及其互作效应分析

陈士亮1,孙亚倩1,邵振启1,李文龙1,孔佑宾1,杜汇1,李喜焕1*,张彩英2*

1.河北农业大学农学院, 华北作物改良与调控国家重点实验室, 河北保定 071001；

2.河北农业大学生命科学学院, 河北保定 071001

收稿日期:2020-12-13 接受日期:2021-03-03 出版日期:2021-08-15 发布日期:2021-08-09
通讯作者: 李喜焕 E-mail: lixihuan@hebau.edu.cn；张彩英 E-mail: zhangcaiying@hebau.edu.cn
作者简介:陈士亮 E-mail: shiliang_chen@yeah.net
基金资助:
河北省现代农业产业技术体系创新团队项目（HBCT2019190203）；

河北省重点研发计划项目（19226356D）。

Analysis of Epistasis QTL and Its Interaction Effects on Controlling Fresh Pod and Seed Related Traits in Soybean

CHEN Shiliang1, SUN Yaqian1, SHAO Zhenqi1, LI Wenlong1, KONG Youbin1, DU Hui1, LI Xihuan1*, ZHANG Caiying2*

1.State Key Laboratory of North China Crop Improvement and Regulation, College of Agronomy, Hebei Agricultural University, Hebei Baoding 071001, China; 2.College of Life Sciences, Hebei Agricultural University, Hebei Baoding 071001, China

Received:2020-12-13 Accepted:2021-03-03 Online:2021-08-15 Published:2021-08-09

摘要/Abstract

摘要： 荚粒是大豆主要的收获器官，直接影响鲜食大豆品种审定和产品出口。然而，荚粒性状由多基因控制，目前主要集中在加性数量性状位点（quantitative trait loci，QTL）发掘方面，对上位性QTL及其互作效应报道甚少。鉴于此，通过鉴定大豆重组自交系（recombinant inbred line，RIL）群体2年4种环境条件下鲜荚和籽粒的长度、宽度、重量等相关性状，发掘控制其上位性QTLs，并研究其互作效应，结果发现，8种测试性状共检测到321对“加性×加性”上位性QTLs，涉及所有染色体，构成复杂的上位性QTLs互作网络，其中包括144对正向效应和177对负向效应QTLs，并以13号染色体分布数量最多；进一步分析发现，存在34对“一因多效”加性×加性上位性QTLs，且加性×加性上位性QTLs间的互作贡献率为1.89%~4.85%，高于其与环境互作贡献率，说明遗传因素为主；上述34对QTLs涉及18条染色体，其中包含定位区间一致的23对“一因多效”QTLs，并有6组上位性QTLs以“一对多”方式发挥功能，16组以“一对一”方式发挥作用。上述结果不仅为实现大豆荚粒性状精准分子遗传改良提供了选择标记，并为进一步揭示大豆荚粒性状分子遗传机制提供了依据。

关键词: 大豆, 荚粒性状, 上位性QTL, 互作网络, 遗传效应

Abstract: Soybeans pod and seed are the most important harvested organs in soybean, which not only directly affect the formation of yield and quality, but also play important roles in fresh variety registration and product export. However, the pod and seed related traits are controlled by multiple minor-effect genes, and the related studies mostly focus on the mining of single additive quantitative trait loci (QTL), while very few researches focus on the epistatic QTLs and their interaction effects. In view of this, eight fresh pod and seed related traits of soybean recombinant inbred line (RIL) population including their length, width and weight etc. were analyzed under four different environments, and the epistatic QTLs and their interaction effects were analyzed in this study. The results showed that there were 321 pairs of ‘additive × additive’ epistatic QTLs detected to associate with these eight related traits, which located on all of the soybean chromosomes, and formed a relative complex epistatic QTLs interaction network. Among these epistatic QTLs, 144 pairs showed positive effects, while 177 pairs showed negative effects, and chromosome 13 had the most quantities of epistatic QTLs. Further analysis revealed that 34 pairs of ‘additive × additive’ epistatic QTLs with pleiotropism effects were identified, and the phenotypic variation explanation (PVE) of ‘additive×additive’ QTLs ranged from 1.89%~4.85%, which were higher than the PVEs of QTLs × environments, and indicated that the genetic factors played the main roles. These 34 pairs of ‘additive × additive’ QTLs were located on 18 chromosomes, among which 23 pairs showed the same mapping regions, respectively. Meanwhile, among these pleiotropism epistatic QTLs, six of them played functions through the ‘single-to-multiple’ QTLs interaction modes, and 16 of them through the ‘single-to-single’ QTLs interaction modes. Thus, these epistatic QTLs not only provided the important selection markers for the molecular genetic improvements of soybean pod and seed traits, but also laid the important foundations for the molecular genetic mechanisms studies of soybean pod and seed related genes in future.

Key words: soybean, pod and seed related traits, epistasis QTL, interaction network, genetic effects

中图分类号:

S565.1

陈士亮, 孙亚倩, 邵振启, 李文龙, 孔佑宾, 杜汇, 李喜焕, 张彩英. 大豆鲜荚籽粒上位性QTL及其互作效应分析[J]. 中国农业科技导报, 2021, 23(8): 25-36.

CHEN Shiliang, SUN Yaqian, SHAO Zhenqi, LI Wenlong, KONG Youbin, DU Hui, LI Xihuan, ZHANG Caiying. Analysis of Epistasis QTL and Its Interaction Effects on Controlling Fresh Pod and Seed Related Traits in Soybean[J]. Journal of Agricultural Science and Technology, 2021, 23(8): 25-36.

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大豆鲜荚籽粒上位性QTL及其互作效应分析

Analysis of Epistasis QTL and Its Interaction Effects on Controlling Fresh Pod and Seed Related Traits in Soybean

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