乳酸乳球菌表达系统的研究进展及其应用

doi:10.13304/j.nykjdb.2023.0244

摘要/Abstract

摘要：

乳酸乳球菌是乳酸菌在异源表达蛋白方面的应用与研究中的一种重要模式菌，由于其公认的安全性、益生特性、无包涵体和内毒素、易于表面显示和细胞外分泌使其成为蛋白异源表达的理想宿主。由于乳酸乳球菌刺激黏膜免疫的特性，其在递呈病毒、细菌抗原等方面得到了广泛应用。阐述了乳酸乳球菌表达系统的相关研究进展，着重介绍了乳酸乳球菌表达异源蛋白的分泌和锚定策略，并总结了对乳酸乳球菌作为细胞工厂在食品、医药，特别是抗原呈递方面的应用，最后对乳酸乳球菌表达系统未来的研究方向进行了展望。

关键词: 乳酸乳球菌, 表达系统, 异源蛋白, 分泌表达, 表面展示

Abstract:

Lactococcus lactis is an important model strain in application and research of lactic acid bacteria in heterologous expression of proteins. L. lactis is an ideal host for heterologous protein expression due to its generally recognized as safe （GRAS） status， probiotic properties， the absence of inclusion bodies and endotoxins， surface display， and extracellular secretion. In view of the properties of L. lactis stimulating mucosal immunity， it has been widely used in presenting viruses and bacterial antigens.The recent research of L. lactis expression system was reviewed， with emphasis on the secretion and anchoring strategies of alloprotein expression of L. lactis， and the application of L. lactis as a microbial cell factory in food， medicine， especially antigen presentation were summarized. Finally， an outlook on the future research direction of the L. lactis expression system were provided.

Key words: Lactococcus lactis, expression system, heterologous proteins, secretion, surface display

中图分类号:

冯哲瀚, 华德平, 梅雪锋, 赵柏萍, 张蕾. 乳酸乳球菌表达系统的研究进展及其应用[J]. 中国农业科技导报, 2025, 27(1): 25-34.

Zhehan FENG, Deping HUA, Xuefeng MEI, Baiping ZHAO, Lei ZHANG. Research Progress and Application of Lactococcus lactis Expression System[J]. Journal of Agricultural Science and Technology, 2025, 27(1): 25-34.

图/表 3

参考文献 81

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启动子 Promoter	蛋白质 Protein	来源 Origin	宿主 Strain	参考文献 Reference
P _PTS-IIC	绿色荧光蛋白mut3a Green fluorescent protein mut3a	水晶果冻水母 Aequoreavictoria	NZ3900	［10］
P₃₂	草酸脱羧酶 Oxalate decarboxylase	枯草芽孢杆菌 Bacillus subtilis	MG1363	［11］
P₄₄	脂肪酶 Lipase	猪葡萄球菌 Staphylococcus hyicus	NZ9000	［12］
P₃₂	细菌素肠霉素P Bacteriocins enterocin P	粪肠球菌P13 Enterococcus faecium P13	NZ9000	［13］
P _tuf	白细胞介素-6 Interleukin-6	小鼠 Murine	IL1403	［14］
P₂₃	烟酰胺单核苷酸合酶 Nicotinamide mononucleotide	土拉弗朗西斯菌 Francisella tularensis	NZ9000	［15］
P₅₉	病毒抗原蛋白 VP2 Virus antigen protein VP2	传染性法氏囊病病毒 Infectious bursal disease virus	MG1363	［16］
P₅₉	葡萄球菌核酸酶 Staphylococcal nuclease	金黄色葡萄球菌 Staphylococcus aureus	MG1363	［17］
P₂₃	溶血素O Listeriolysin O	李斯特菌 Listeria monocytogenes	MG1363	［18］
P₁	脲酶亚基B Urease subunit B	幽门螺杆菌 Helicobacter pylori	MG1363	［19］

启动子 Promoter	蛋白质 Protein	来源 Origin	宿主 Strain	参考文献 Reference
P _PTS-IIC	绿色荧光蛋白mut3a Green fluorescent protein mut3a	水晶果冻水母 Aequoreavictoria	NZ3900	［10］
P₃₂	草酸脱羧酶 Oxalate decarboxylase	枯草芽孢杆菌 Bacillus subtilis	MG1363	［11］
P₄₄	脂肪酶 Lipase	猪葡萄球菌 Staphylococcus hyicus	NZ9000	［12］
P₃₂	细菌素肠霉素P Bacteriocins enterocin P	粪肠球菌P13 Enterococcus faecium P13	NZ9000	［13］
P _tuf	白细胞介素-6 Interleukin-6	小鼠 Murine	IL1403	［14］
P₂₃	烟酰胺单核苷酸合酶 Nicotinamide mononucleotide	土拉弗朗西斯菌 Francisella tularensis	NZ9000	［15］
P₅₉	病毒抗原蛋白 VP2 Virus antigen protein VP2	传染性法氏囊病病毒 Infectious bursal disease virus	MG1363	［16］
P₅₉	葡萄球菌核酸酶 Staphylococcal nuclease	金黄色葡萄球菌 Staphylococcus aureus	MG1363	［17］
P₂₃	溶血素O Listeriolysin O	李斯特菌 Listeria monocytogenes	MG1363	［18］
P₁	脲酶亚基B Urease subunit B	幽门螺杆菌 Helicobacter pylori	MG1363	［19］

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