不同菜用甘薯品种茎尖代谢产物鉴定及途径分析

doi:10.13304/j.nykjdb.2024.0371

中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (2): 62-69.DOI: 10.13304/j.nykjdb.2024.0371

不同菜用甘薯品种茎尖代谢产物鉴定及途径分析

张奕萱¹(), 李慧峰²^,³(), 黄咏梅²^,³, 李彦青²^,³, 滑金锋²^,³, 银捷²^,³, 陈天渊²^,³, 肖冬¹, 莫云川¹()

^1.广西大学农学院，广西农业环境与农产品安全重点实验室，南宁 530004
^2.广西壮族自治区农业科学院玉米研究所，南宁 530007
^3.广西壮族自治区农业科学院旱粮作物研究所，南宁 530007

收稿日期:2024-05-11 接受日期:2024-09-18 出版日期:2025-02-15 发布日期:2025-02-14
通讯作者: 李慧峰,莫云川
作者简介:张奕萱 E-mail：468945225@qq.com
基金资助:
国家自然科学基金项目(32060419);国家甘薯产业技术体系建设项目(CARS-10-SYZ14);广西农业科学院基本科研业务专项(桂农科2021YT022)

Separation and Identification of Metabolites and Metabolic Pathway Analysis in Different Vegetable Sweet Potato

Yixuan ZHANG¹(), Huifeng LI²^,³(), Yongmei HUANG²^,³, Yanqing LI²^,³, Jinfeng HUA²^,³, Jie YIN²^,³, Tianyuan CHEN²^,³, Dong XIAO¹, Yunchuan MO¹()

^1.Guangxi Key Laboratory of Agricultural Environment and Agricultural Product Safety，College of Agronomy，Guangxi University，Nanning 530004，China
^2.Maize Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007，China
^3.Dryland Crops Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007，China

Received:2024-05-11 Accepted:2024-09-18 Online:2025-02-15 Published:2025-02-14
Contact: Huifeng LI,Yunchuan MO

摘要/Abstract

摘要：

为研究代谢产物对甘薯生长发育的影响，以甘薯茎尖为研究对象，利用液相色谱-质谱联用（liquid chromatograph-mass spectrometer，LC-M）分离鉴定南宁菜、福菜薯18号、桂菜薯1号、桂菜薯2号、福薯7-6和台农71共6种菜用甘薯的差异代谢产物，探讨代谢途径的差异。结果表明，6个甘薯品种共得到差异代谢物4 210种，其中上调2 229种，下调1 981种，表明不同甘薯品种间存在显著差异。差异代谢物涉及的代谢途径主要包括初级胆汁酸生物合成、吲哚生物碱生物合成、黄酮和黄酮醇生物合成和色氨酸代谢，且不同品种间差异代谢物的积累和代谢途径也存在较大差异。因此，推测差异代谢物的积累和代谢途径的差异对甘薯品质起着关键作用。以上研究结果为进一步分析不同品种甘薯品质差异的机制奠定了基础。

关键词: 菜用甘薯, 茎尖, 代谢产物, 代谢途径

Abstract:

To study the effects of metabolites on the growth and development of vegetable sweet potato， the stem tips of sweet potatoes were as materials， and liquid chromatograph-mass spectrometer （LC-MC） was employed to identify and separate metabolic products of 6 sweet potato cultivars including Nanningcai， Fucaishu 18， Guicaishu 1， Guicaishu 2， Fushu 7-6 and Tainong 71. The differences of metabolic pathways were further discussed. The results revealed a total of 4 210 differential metabolites were detected across 6 sweet potato cultivars， with 2 229 up-regulated and 1 981 down-regulated， which showed that there were significant differences among different sweet potato cultivars. The primary metabolic pathways associated with differential metabolism included primary bile acid biosynthesis， indole alkaloid biosynthesis， flavonoid and flavonol biosynthesis and tryptophan metabolism. The accumulation of differential metabolites and their respective metabolic pathways among different varieties had significant differences. Consequently， it was hypothesized that the differences in both the accumulation and metabolism of these metabolites played a crucial role in sweet potato quality. Above results provided reliable basis for understanding the mechanisms of differences on quality of sweet potato.

Key words: vegetable sweet potato, stem tip, metabolite, metabolic pathway

中图分类号:

S531

张奕萱, 李慧峰, 黄咏梅, 李彦青, 滑金锋, 银捷, 陈天渊, 肖冬, 莫云川. 不同菜用甘薯品种茎尖代谢产物鉴定及途径分析[J]. 中国农业科技导报, 2025, 27(2): 62-69.

Yixuan ZHANG, Huifeng LI, Yongmei HUANG, Yanqing LI, Jinfeng HUA, Jie YIN, Tianyuan CHEN, Dong XIAO, Yunchuan MO. Separation and Identification of Metabolites and Metabolic Pathway Analysis in Different Vegetable Sweet Potato[J]. Journal of Agricultural Science and Technology, 2025, 27(2): 62-69.

图/表 4

图1 6个甘薯品种的主成分分析A：正离子模式；B：负离子模式。_1~_6表示6次生物学重复

Fig. 1 Principal component analysis of 6 sweet potato varietiesA： NEG ion model； B： POS ion model. _1~_6 indicate 6 biological replicates

图2 OPLS-DA模型置换检验

Fig. 2 OPLS-DA model displacement test

表1 不同甘薯品种间的差异代谢物

Table 1 Differential metabolites among different varieties of sweet potato

品种对比 Variety comparison	数量Number
品种对比 Variety comparison	总计 Total	上调 Up-regulation	下调 Down-regulation
FCS18 vs NNC	314	189	125
GCS1vs NNC	304	166	138
GCS1 vs FCS18	253	138	115
GCS2 vs NNC	300	148	152
GCS2 vs FCS18	311	155	156
GCS2 vs GCS1	292	132	160
FS7-6 vs NNC	287	162	125
FS7-6 vs FCS18	254	116	138
FS7-6 vs GCS1	276	126	150
FS7-6 vs GCS2	315	154	161
TN71 vs NNC	331	185	146
TN71 vs FCS18	272	146	126
TN71 vs GCS1	209	119	90
TN71 vs GCS2	209	132	77
TN71vs FS7-6	283	162	122

图3 不同甘薯品种间差异代谢物的KEGG富集

Fig. 3 KEGG enrichment of differential metabolites among different varieties of sweet potato

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不同菜用甘薯品种茎尖代谢产物鉴定及途径分析

Separation and Identification of Metabolites and Metabolic Pathway Analysis in Different Vegetable Sweet Potato

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