豇豆胰蛋白酶抑制剂基因（cpti）及其在抗虫转基因作物中的应用

›› 2008, Vol. 10 ›› Issue (1): 18-27.

豇豆胰蛋白酶抑制剂基因（cpti）及其在抗虫转基因作物中的应用

徐鸿林[1] 翟红利[1] 王锋[2] 朴建华[3] 杨晓光[3] 朱祯[1]

[1]中国科学院遗传与发育生物学研究所,北京100101 [2]福建省农业科学院生物技术研究所,福州350003 [3]中国疾病预防控制中心营养与食品安全所,北京100021

收稿日期:2007-12-07 修回日期:2007-12-26 出版日期:2008-02-15 发布日期:2009-07-30
通讯作者: 朱祯，研究员，博士生导师，从事生物技术、植物基因组学、转基因农作物育种及安全性研究。Tel：010-64852890；E-mail：zzhu@genetics．ac．cn
作者简介:徐鸿林|工程师|从事分子生物学研究。E-mail：hlxu@genetics．ac．cn。
基金资助:
国家“973”计划项目（2007CB109201）,中国科学院知识创新工程重大项目（KSCX1-YW-03）和中荷科学战略联盟项目（2004CB720406）资助.

Cowpea Trypsin Inhibitor Gene （cpti） and its Application |in Insect Resistance Transgenic plants

XU Hong-lin, ZHAI Hong-li, WANG Feng, PIAO Jian-hua, YANG Xiao-guang| ZHU Zhen

1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101 |2. Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003 |3. Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021| China

Received:2007-12-07 Revised:2007-12-26 Online:2008-02-15 Published:2009-07-30

摘要/Abstract

摘要：

豇豆胰蛋白酶抑制剂（cowpea trypsin inhibitor,CpTI）来自豇豆的可食用部分,由于其具有抗虫谱广且昆虫不易对其产生耐受性的特点,cpti基因被作为抗虫植物基因工程一个重要的候选基因。目前,cpti基因已经被转到烟草、水稻、棉花和番茄等多种作物中。本文介绍了CpTI的特性、抗虫机理、基因工程研究进展,以及转cpti基因水稻的安全性研究,并对蛋白酶抑制剂的分类和安全性问题做了相关介绍。CpTI在分类上属于Bowmun-Birk家族,大量医学研究表明,该家族蛋白质对于癌症的预防和辅助治疗都有积极作用,对人体不存在任何危害。

关键词: 豇豆胰蛋白酶抑制剂（CpTI）转基因作物水稻生物安全性

Abstract:

Cowpea trypsin inhibitor （ CpTI ） is derived from the edible part of cowpea, cpti gene is an important candidate gene in plant genetic engineering for its broad insect resistance spectrum, and ability of delaying the insect resistance. At present, cpti gene has been transformed into tobacco, rice, cotton and tomato etc. This paper intro- duces the characteristics of CpTI, its insect resistance mechanism and research progress in gene engineering, including the research and safety evaluation of cpti transgenic rice. CpTI belongs to the Bowman-Birk family, which plays an active role in preventing cancer occurrence and as an assistant therapy, and is applied as an anticarcinogenic agent without any toxicity to human beings.

Key words: cowpea trypsin inhibitor （CpTI） , transgenic crop, rice, bio-safety

中图分类号:

Q789 S433.1

徐鸿林,翟红利,王锋,朴建华,杨晓光,朱祯. 豇豆胰蛋白酶抑制剂基因（cpti）及其在抗虫转基因作物中的应用[J]. , 2008, 10(1): 18-27.

XU Hong-lin, ZHAI Hong-li, WANG Feng, PIAO Jian-hua, YANG Xiao-guang| ZHU Zhen. Cowpea Trypsin Inhibitor Gene （cpti） and its Application |in Insect Resistance Transgenic plants[J]. , 2008, 10(1): 18-27.