关键词:  组织培养;污染;抗菌肽

Abstract:

The treatment of peeled cotton seeds by 0.1% mercuric chloride for 5 min, washed with sterile water 3~5 times, and then

immersed in 0.12 mg/mL antibacterial peptide liquid, was effective in preventing most common fungal contamination of the

cotton tissue culture, and did not effect the development of cotton roots. The using of antibacterial peptide in callus

induction stage showed no impact on cotton callus.The using of antibacterial peptide in the tobacco tissue culture showed no

impact on callus,shoots and shoots regeneration. Individual treatment by antibacterial peptide for the bacteria, the

bactericidal effect was less than that by cephalosprin, but the joint treatment of antibacterial peptide and cephalosprin

could prevent  bacterial contamination effectively. In this preliminary study antibacterial peptide could be  applied in

cotton and  tobacco tissue culture present good performances in fungal and bacterium contamination control.

Key words: tissue culture, contamination, antibacterial peptide