关键词: 植物 磷酸化 磷酸化蛋白质组 质谱 分离与鉴定

Abstract:

Protein phosphorylation is the most important mode of post-translational modifications. Protein phosphorylation and dephosphorylation play a critical regulation function in the whole process of vital movement. The major objectives of the present phosphoproteomics include the isolation of global phosphorylation protein in the cell, the generation of global phosphorylation protein map, and the identification of phosphorylated site on phosphorylation protein, and then regulation functional analysis of phosphorylation and dephosphorylation in the life process. On the basis of introducing the research approaches and the latest progress on plant phosphoproteomics, this paper also looks into the prospects of phosphoproteomics.

Key words: plant , phosphorylation , phosphoproteome , mass spectrometry , isolation and identification