关键词: 叶绿素含量 可控环境 光合速率 多糖含量

Abstract:

The effect of photoperiod on growth and development of Dendrobium officinale in vitro was studied under controlled environment in a closed plant factory with artificial lighting. Single explant each with 300 mg fresh mass of D. officinale were planted in five treatments with photoperiods of 6, 9, 12, 15, and 18h·d^-1 under controlled environment at air temperature of 22 ± 1℃, relative humidity of 65 ± 5%, lighting intensity of 68 ± 9 μmol · m^^-1· s^-1, and COz concentration of 800 ± 50 μmol· mol -l in photoperiock The net photosynthetic rate, chlorophyll content, dry mass, and buds in treatment of photoperiod of 12 h·d^-1were higher than that in other treatments, and resulted in greater growth and reproduction for D. officinale producction in vitro after the explants were cultured 92 days. Therefore, the photoperiod of 12 h·d^-1 for D. officinale production in vitro is suitable in tthe closed plant factory with artificial lighting.

Key words: chlorophyll content , controlled environment , photosynthetic rate , polysaceharide content