细菌性条斑病侵染水稻抗性相关蛋白研究

doi:10.3969/j.issn.1008-0864.2010.05.11

›› 2010, Vol. 12 ›› Issue (5): 62-67.DOI: 10.3969/j.issn.1008-0864.2010.05.11

细菌性条斑病侵染水稻抗性相关蛋白研究

李东霄1,2,张国广2,郭立佳2,李敏2,毛倩2,汪卫2,陈亮2

(1.河南科技学院, 河南新乡 453003|2.厦门大学生命科学学院, 福建厦门 361005)

收稿日期:2010-07-05 修回日期:2010-08-14 出版日期:2010-10-15 发布日期:2010-10-12
通讯作者: 陈亮,教授,博士,主要从事农业生物技术研究。Tel:0592-2186050; E-mail:chenlg@xmu.edu.cn
作者简介:李东霄,博士研究生,主要从事农业生物技术研究。
基金资助:
国家863计划项目(2007AA10Z132);国家重大科技专项(2008ZX08001-001;2009ZX08009-045B);教育部重点项目(重点01102)资助。

Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak, Xanthomonas Oryzae pv. Oryzicola

LI Dong-xiao1,2, ZHANG Guo-guang2, GUO Li-jia2, LI Min2, MAO Qian2, WANG Wei2, |CHEN Liang2

(1.Henan Institute of Science and Technology, Henan Xinxiang 453003|2.School of Life Sciences, Xiamen University, Fujian Xiamen 361005, China)

Received:2010-07-05 Revised:2010-08-14 Online:2010-10-15 Published:2010-10-12

摘要/Abstract

摘要：

水稻细菌性条斑病由Xanthomonas oryzae pv. oryzicola引起,是目前威胁亚洲稻区水稻生产的重要病害之一。以水稻品种9311为研究对象, 通过差异蛋白质组学方法,研究了病菌侵染48 h后水稻叶片的差异表达蛋白质,通过分析比对、质谱分析以及数据库检索,从中选择了7个上调表达的鉴定蛋白,包括4个水稻LRK类基因,2个NBS-LRR 类基因和1个PR-10基因家族成员基因。并设计相应的PCR引物,从水稻cDNA 中获得相关基因片段做探针进行Northern杂交分析,结果显示,上述基因在接种病原菌12 h或48 h后表达量增加,表明这些蛋白质参与了抗病反应。

关键词: 细菌性条斑病;水稻;蛋白组学;抗病;Northern杂交

Abstract:

Rice bacterial leaf streak (BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola (Xooc) is one of the major diseases in Asia. In this paper, the protein differently expressed after infected for 48 h by BLS was studied with rice 9311 as material through proteomic approach. Based on the result of protein blast, fingerprints and data search analysis, seven identified proteins up-regulated were selected including four LRK like genes, two NBS-LRR type genes and one PR-10 gene. Relevant primers were designed to amplify the genes from rice cDNA as probe for Northern blotting. The results showed that the mRNA levels of these genes increased significantly after infected by BLS for 12 h and 48 h, indicating these proteins have participated in disease resistance.

Key words: bacterial leaf streak, rice, proteomics, disease resistance, Northern blotting

中图分类号:

S435.111.4

李东霄1,2,张国广2,郭立佳2,李敏2,毛倩2,汪卫2,陈亮2. 细菌性条斑病侵染水稻抗性相关蛋白研究[J]. , 2010, 12(5): 62-67.

LI Dong-xiao1,2, ZHANG Guo-guang2, GUO Li-jia2, LI Min2, MAO Qian2, WANG Wei2, . Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak, Xanthomonas Oryzae pv. Oryzicola[J]. , 2010, 12(5): 62-67.

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细菌性条斑病侵染水稻抗性相关蛋白研究

Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak, Xanthomonas Oryzae pv. Oryzicola

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