关键词: 籼稻;遗传转化;农杆菌介导;影响因素

Abstract:

Recently Agrobacterium-mediated transformation of rice has been widely used in japonica transformation. However, the transformation of indica rice is still difficult, mainly because the induction of embryogenic callus is not routine and the rate of plant regeneration from transformed calli is low. There are big genetic differences between these 2 subspecies, and indica rice is particularly genotype-dependent. This paper briefly reviewed the history of agrobacterium-mediated transformation of indica rice and recent progress achieved. It also introduced various factors affecting indica rice transformation, analyzed problems existing in its research and provided strategy to improve the efficiency of transformation. The establishment of high efficient indica rice transformation system will accelerate the process of applying transformation technology in rice breeding programs and promote the studies on rice functional genomics.

Key words: Indica rice, genetic transformation, Agrobacterium-mediated, influencial factors