Abstract:

In order to develop a new pathway to utilize kelp waste, Hypsizigus marmoreus was incubated in medium mainly composed of kelp waste and products from fermentation were investigated with antioxidant assays. The results showed that mushroom could grow in this medium and produce abundant mycelia. Antioxidant assays indicated that the total antioxidant capacity (T-AOC) of the supernatant and extract of mycelia were 0.23±0.02 U/mg and 0.77±0.01 U/mg, which were 43.8% and 18.5% higher than those of the medium; and also significantly higher than that of the chemical antioxidant BHT. Furthermore, the supernatant of fermentation broth exhibited potent capacity for hydroxyl radical scavenging and superoxide anion radical scavenging, as their EC50 values were 0.43 mg/mL and 26.02 mg/mL. The study showed that the compounds with potent antioxidant activity produced during the fermentation mainly distribute inside of the mycelia, and the compounds with potent capacity for hydroxyl radical scavenging mainly distribute in the supernatant.

Key words: kelp waste, Hypsizigus marmoreus, antioxidant, hydroxyl radical, superoxide anion radical

摘要：

为了开发海带废渣资源化利用的新途径,以真姬菇为供试菌株,对海带废渣进行液体发酵,并研究发酵产物的抗氧化活性。结果表明真姬菇能以海带渣为营养基质,进行生长发酵,并产生有效的抗氧化活性物质。抗氧化试剂盒检测显示发酵上清液总抗氧化活性为0.23±0.02 U/mg,比未发酵的海带废渣高43.8%,其发酵产物提取液的总抗氧化活性为0.77±0.01 U/mg,比未发酵的海带废渣高18.5%,且均高于化学抗氧化剂BHT。发酵上清液还具有良好的羟自由基清除能力（EC50=0.43 mg/mL）和一定的超氧阴离子自由基清除能力（EC50=26.02 mg/mL）。分析结果表明发酵过程中产生的抗氧化活性物质主要存在于菌丝体内部,而具有羟自由基清除能力的活性物质则游离于发酵上清液。

关键词: 海带废渣;真姬菇;抗氧化;羟自由基;超氧阴离子自由基