›› 2014, Vol. 16 ›› Issue (1): 157-162.DOI: 10.13304/j.nykjdb.2013.240

• 方法与技术创新 • 上一篇    下一篇

甘薯渣酶法生产葡萄糖的技术研究

吴允山1,郭军2,严共鸿3,魏东旺1*,葛福祥1, 尚建丽1,姚爱萍1,潘磊1,田健2   

  1. (1.山东宏河圣齐生物工程有限公司| 山东 济宁 273517; 2.中国农业科学院生物技术研究所| 北京 100081; 3.匹兹堡大学| 美国 匹兹堡 15213)
  • 出版日期:2014-02-15 发布日期:2014-02-17
  • 通讯作者: 魏东旺,工程师,博士,研究方向为生物工程。E\|mail: dwwei@tom.com
  • 作者简介:吴允山|高级工程师|主要从事生物质转化和催化工作。E\|mail: wys1939@sina.cn。
  • 基金资助:

    山东省第二批技术创新项目(201220508004)资助。

Studies on Technique of Producing Glucose from Sweet Potato Residue by Enzymes

WU Yun\|shan1, GUO Jun2, YAN Gong\|hong3, WEI Dong\|wang1*, GE Fu\|xiang1, SHANG Jian\|li1, YAO Ai\|ping1, PAN Lei1, TIAN Jian2   

  1. (1.Shandong Honghe Sunkeen Biotechnology Co., Ltd, Shandong Jining 273517|2.Biotechnology Research Institute| Chinese Academy of Agricultural Sciences| Beijing 100081, China; 3.University of Pittsburgh| Pittsburgh 15213, USA)
  • Online:2014-02-15 Published:2014-02-17

摘要:

甘薯渣是以甘薯为原料生产淀粉后的残渣,其中含淀粉50%左右。通过在甘薯渣粉中加入耐高温α\|淀粉酶、糖化酶和纤维素酶进行处理,然后分别对其进行保温和灭酶处理,再对糖化醪进行固液分离,浓缩得到液糖产品。经高效液相色谱(HPLC)分析发现其成分大部分为葡萄糖。利用正交试验初步得到了酶解的最佳工艺条件:料水比6%,淀粉酶加量12 U/g干物质,液化时间为60 min,糖化酶加量300 U/g干物质,纤维素酶加量0.5 U/g干物质,糖化时间为24 h。该方法工艺简单,专一性强,所得产品品质好,收率高,可有效解决甘薯渣严重污染环境的问题,且具有良好的工业应用前景。

关键词: 甘薯渣;结合淀粉;酶法;葡萄糖

Abstract:

Sweet potato residue is the by\|product of sweet potato starch production. It contains about 50% starch. In this study, the alpha\|amylase, glucoamylase, and cellulase were used to hydrolyze the sweet potato residue, and then were inactivated by the thermo treatment. The sugar products were separated and concentrated. Analyzed by the high performance liquid chromatography (HPLC), most component of the product is glucose. The condition of enzyme hydrolysis was optimized with the orthogonal experiment, and the results are as follows: the material content is 6 percent, the amount of amylase is 12 U/g dry matter, liquefaction time is 60 min, the amount of glucoamylase is 300 U/g dry matter, the amount of cellulase is 0.5 U/g dry matter, saccharification time is 24 h. Because of its simple craft and its product with good quality and high quantity, this technique can help to solve serious environmental pollution caused by sweet potato residue, may have good industrial applications prospects.

Key words: sweet potato residue, bound starch, enzyme, glucose

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