耐盐碱抗烟草黑胫病木霉菌株的筛选与鉴定

doi:10.13304/j.nykjdb.2013.450

›› 2014, Vol. 16 ›› Issue (3): 81-89.DOI: 10.13304/j.nykjdb.2013.450

耐盐碱抗烟草黑胫病木霉菌株的筛选与鉴定

崔西苓1,2,李世贵1,2,杨佳2,樊祥臣2,顾金刚1,2*

(1.中国农业科学院农业资源与农业区划研究所, 农业部农业微生物资源收集与保藏重点实验室, 北京 100081|2.中国农业微生物菌种保藏管理中心, 北京 100081)

收稿日期:2013-08-30 修回日期:2013-12-31 出版日期:2014-06-15 发布日期:2014-04-30
通讯作者: 顾金刚，副研究员。研究领域为真菌资源农业应用。 E-mail:gujingang@caas.cn
作者简介:崔西苓|硕士研究生|研究方向为农业微生物资源。E-mail：cxl19891113@163.com。
基金资助:
国家“十二五”科技支撑计划项目(2011BAD11B05);四川省烟草专卖局(201202006)资助。

Screening and Identification of Saline\|alkali Tolerant and Tobacco Black Shank Resistant Trichoderma

CUI Xi-ling1,2, LI Shi\|gui1,2, YANG Jia2, FAN Xiang-chen2, GU Jin-gang1,2*

(1.Key Laboratory of Microbial Resources, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing |100081|2.National Agricultural Microbial Strains Preservation and Management Center, Beijing 100081, China)

Received:2013-08-30 Revised:2013-12-31 Online:2014-06-15 Published:2014-04-30

摘要/Abstract

摘要：

采用稀释平板涂布法，从天津、河北地区采集的48份盐碱土中分离筛选得到20株耐盐碱木霉菌株，在含8% NaCl，pH 8.0的PDA培养基中培养7 d，菌落直径达1.0~5.9 cm。通过平板对峙培养和发酵液活性测定得到6株对Phytophthora nicotianae ACCC38065具有较好拮抗作用的菌株。其中，菌株28TJ19对Phytophthora nicotianae ACCC38065的拮抗效果最好，平板对峙和发酵液抑制率分别为52.8%、71.6%，其次为菌株28HB14(41.7%, 76.2%)、29HB16(41.7%, 67.3%)。利用形态学观察、ITS序列分析以及TEF序列分析对这6株菌株进行分类鉴定，4株菌株鉴定为哈茨木霉(Trichoderma harzianum)，另外2株为长枝木霉(Trichoderma longibrachiatum)。经盆栽试验发现，这6株菌对烟草黑胫病均具有不同程度的防治效果，菌株29TJ04、29HB16的防效最好，达100%。

关键词: 耐盐碱；抗烟草黑胫病；木霉菌；筛选鉴定

Abstract:

In this study, 20 saline\|alkali tolerant Trichoderma strains were isolated and screened from 48 saline\|alkali soil samples collected in Tianjin, Hebei Province by dilution plate coating method. When they were cultured on PDA medium at 8% NaCl concentration and pH 8.0 for 7 days, colony diameter went up to 1.0~5.9 cm. Phytophthora nicotianae ACCC38065 was used as pathogen to evaluate the disease\|resistant effect of Trichoderma selected. Six strains were selected, which have better antagonistic effect on Phytophthora nicotianae ACCC38065. Of which, strain 28TJ19 has the best antagonism effect. Tablet confrontation and inhibition rate were 52.8% and 71.6%, respectively, followed by strain 28HB14(41.7%, 76.2%)、29HB16(41.7%, 67.3%). These six strains were classified and identified by morphological observation, ITS sequence analysis and TEF sequence analysis. Four of them were identified as Trichoderma harzianum. The other two strains were Trichoderma longibrachiatum. The results of greenhouse test indicated that all these 6 strains had bio\|control effect of different degrees on tobacco blank shank. Among them the effect of strain 29TJ04 and 29HB16 were the best, which could reach 100%.

Key words: saline\, alkali tolerance, tobacco black shank resistance, Trichoderma, screening and identification

中图分类号:

S435.72

崔西苓1,2,李世贵1,2,杨佳2,樊祥臣2,顾金刚1,2*. 耐盐碱抗烟草黑胫病木霉菌株的筛选与鉴定[J]. , 2014, 16(3): 81-89.

CUI Xi-ling1,2, LI Shi-gui1,2, YANG Jia2, FAN Xiang-chen2, GU Jin-gang1,2*. Screening and Identification of Saline\|alkali Tolerant and Tobacco Black Shank Resistant Trichoderma[J]. , 2014, 16(3): 81-89.

耐盐碱抗烟草黑胫病木霉菌株的筛选与鉴定

Screening and Identification of Saline\|alkali Tolerant and Tobacco Black Shank Resistant Trichoderma

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