关键词: 芥子油苷；芥蓝；提取温度；甲醇浓度；提取时间；物料比

Abstract:

Taking the orthogonal test design, this paper studied the effect of different extraction factors (extract temperature, methanol concentrations of extract solution, extract time, and ratios between sample to be tested and extract solution) on glucosinolates (GSs) extraction the total content of GSs, total aliphatic GSs, and total indolyl GSs from bolting stems of Chinese kale (Brassica alboglabra Bailey). Sample/solution ratio and extract temperature had significant influence on the total content of GSs, total aliphatic GSs, and total indolyl GSs (P<0.05). While, methanol concentration and extract time had no significant influence on Glucosinolate extraction yield (P>0.05). Considering the extraction effect and cost, we suggested the optimal extract temperature, ratio of sample to extract solution, methanol concentrations of extract solution, and extract time are 75℃, 1∶45(g∶mL), 70% and 5 min, respectively. Under the optimal condition, the total content of GSs, total aliphatic GSs, and total indolyl GSs are 8.31 μmol/g DW, 7.58 μmol/g DW, and 0.73 μmol/g DW, respectively.

Key words: glucosinolates(GSs), Chinese kale(Brassica alboglabra Bailey), extract temperature, methanol concentration, extract time, ratio of sample to extract solution