关键词: SELEX技术;单链DNA;链霉亲和素磁珠;不对称PCR

Abstract:

In order to establish a simple and effective method for preparing single\|stranded DNA in SELEX screening process, this study optimized the experimental conditions for preparing the streptavidin\|conjugated magnetic nanoparticles, decided the best time and saturation of symmetric PCR product binding with streptavidin\|conjugated magnetic nanoparticles, verified the influence of different NaOH concentration on desmolysis effect, and established the ssDNA preparation system applied to SELEX technology. The purity and recovery efficiency of ssDNA obtained by established method in this experiment and traditional asymmetric PCR method was compared using fluorescence and polyacrylamide gel electrophoresis. The result showed that under optimal experimental condition the method established in this experiment was significantly better than asymmetric PCR method.

Key words: SELEX technology, ssDNA, streptavidin\, conjugated magnetic nanoparticles, asymmetrical PCR