关键词: 玉米；YABBY家族；发育；逆境

Abstract:

YABBY gene family is a class of transcription factors widely existed in spermatophyte, and involved in plant embryonic development, lateral organ differentiation, and stress response, and other biological processes. Maize YABBY family transcription factors consisted of 13 members, containing a zinc finger-like domain in N-terminal and a helix-loop-helix domain in C-terminal. qRT-PCR showed that the expression of YABBY family presented obvious tissue specificity and was higher in reproductive organ than that in nutritive organs except ZmYAB1. ZmYAB5, ZmYAB7, ZmYAB9 and ZmYAB13 had a relatively high overall expression level, whereas ZmYAB3, ZmYAB4 and ZmYAB8 were barely. When we analyzed maize YABBY family’s response reaction to stress, we discovered that in 4℃environment, the expression of ZmYAB1, ZmYAB5 and ZmYAB13 decreased sharply within 3 h treatment, whereas that of ZmYAB9 increased during 24 h treatment. For NaCl treatment, the expression of whole YABBY family increased firstly and then decreased gradually. For PEG treatment, expression of the whole YABBY family decreased. For SA treatment, the expression of ZmYAB5 and ZmYAB13 increased remarkably, that of ZmYAB1, ZmYAB6, ZmYAB7 and ZmYAB9 increased significantly within 3h treatment, and then fall back, whereas that of ZmYAB9 decreased when treated over 12 h. For ABA treatment, expression of ZmYAB1, ZmYAB2, ZmYAB6 and ZmYAB9 increased with different degrees, whereas that of ZmYAB7 and ZmYAB10 showed obvious cut trend when treated over 6 h. Expression of ZmYAB5 and ZmYAB13 increased for the overall trend. All these data confirm the function of all YABBY family members in maize.

Key words: maize, YABBY family, development, stress