关键词: 紫花苜蓿；胚胎再生途径；丛生芽诱导

Abstract:

The establishment of alfalfa high frequency regeneration system is helpful to promote the studies on its genetic transformation. Taking Longdong, Algonquin and Gannong 4 alfalfa (Medicago sativa L.) as experimental materials, this study compared the effects by somatic embryo regeneration and multiple shoots clumps regeneration on alfalfa regeneration cycle and frequency. Meanwhile, different explants and growth regulators were matched to realize further optimization of these 2 regeneration pathways. The results showed that the regeneration effect of Gannong 4 was better 3 alfalfa species and hypocotyl was higher. In callus induction phase adding 2 mg/L 2,4-D and 1 mg/L, in embryoid induction phase adding 1 mg/L KT and 0.5 mg/L NAA, better regeneration effect could be achieved. Adding 1mg/L 6-BA and 1mg/L TDZ in culture medium could get the best regeneration via multiple shoots. Regeneration via multiple shoots clumps pathway had shown excellent superiority. Its regeneration time was only 10 weeks, which was an alternative way for the establishment of alfalfa regeneration system.

Key words: Medicago sativa L., somatic embryogenesis regeneration, multiple shoot induction