猪繁殖与呼吸综合征疫苗室温保存工艺的研究

doi:10.13304/j.nykjdb.2015.065

›› 2015, Vol. 17 ›› Issue (3): 175-181.DOI: 10.13304/j.nykjdb.2015.065

• 方法与技术创新 • 上一篇

猪繁殖与呼吸综合征疫苗室温保存工艺的研究

郝政林1,2,吕芳1,卢宇1,赵艳红1,王丽丽1,2,侯继波1*,芮荣2

(1.江苏省农业科学院国家兽用生物制品工程技术研究中心, 南京 210014|2.南京农业大学动物医学院, 南京 210014)

收稿日期:2015-02-02 修回日期:2015-04-30 出版日期:2015-06-15 发布日期:2015-05-16
通讯作者: 侯继波，研究员，博士，主要从事兽用生物制品研究。E-mail：houjibo@jaas.ac.cn
作者简介:郝政林|硕士研究生|研究方向兽用疫苗新剂型。E-mail：luyu@jaas.ac.cn。
基金资助:
江苏省农业科技自主创新项目\[CX（14）2085\]，公益性（农业）行业基本科研业务费专项（201303046）资助。

Studies on Room Temperature Preservation Technology of Porcine Reproductive and Respiratory Syndrome Vaccine

HAO ZHENG-lin1,2, LV Fang1, LU Yu1, ZHAO Yan-hong1, WANG Li-li1,2, HOU Ji-bo1*, RUI Rong2

(1.National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014|2.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China)

Received:2015-02-02 Revised:2015-04-30 Online:2015-06-15 Published:2015-05-16

摘要/Abstract

摘要：

为了提高猪繁殖与呼吸综合征（PRRS）弱毒活疫苗的耐热性能，用蔗糖、胰蛋白胨、EDTA、乳糖等成分配伍成不同组方，对PRRS病毒进行梯度真空泡沫干燥。37℃ 10 d耐老化试验结果表明，组方T28C-1、T28C-4、T28C-5和T28C-7的病毒损失均＜0.5 Lg；疫苗长期保存期试验结果表明，T28C-4、T28C-5在37℃可保存4个月，25℃及自然条件下可保存5个月以上；将37℃、25℃及4℃下保存3个月的T28C-4干燥疫苗免疫20日龄仔猪，免后2周ELISA抗体水平合格且与商品苗趋势相当。试验结果表明PRRSV泡沫干燥疫苗有很好的耐热效果。

关键词: 泡沫干燥；室温保存；猪繁殖与呼吸综合征病毒

Abstract:

To improve the heat resistance of PRRS attenuated live vaccine, several formulas composed of sucrose, tryptone, EDTA, lactose and other protectants were dried by vacuum foam along with porcine reproductive and respiratory syndrome virus. The results of aging resistance test at 37℃ for 10 d indicated that the virus titer losts were no more than 0.5 Lg in 4 formulations of T28C-1, T28C-4, T28C-5, and T28C-7. The results of vaccine long term preservation test indicated that formula T28C-4 and T28C-5 could be kept at 37℃ for 4 months, while at 25℃ and under natural conditions they could be kept for over 5 months. Besides，after preserved at 37℃、25℃ and 4℃ for 3 months, the dried vaccine T28C-4 was inoculated on piglet at 20 days of age, and 2 weeks later, the ELISA titer were all qualified and had the similar level to the commercial vaccines of lyophilization dosage.

Key words: foam dry, room temperature preservation, PRRSV

中图分类号:

S852.65

郝政林1,2,吕芳1,卢宇1,赵艳红1,王丽丽1,2,侯继波1*,芮荣2. 猪繁殖与呼吸综合征疫苗室温保存工艺的研究[J]. , 2015, 17(3): 175-181.

HAO ZHENG-lin1,2, LV Fang1, LU Yu1, ZHAO Yan-hong1, WANG Li-li1,2, HOU Ji-bo1*, . Studies on Room Temperature Preservation Technology of Porcine Reproductive and Respiratory Syndrome Vaccine[J]. , 2015, 17(3): 175-181.

猪繁殖与呼吸综合征疫苗室温保存工艺的研究

Studies on Room Temperature Preservation Technology of Porcine Reproductive and Respiratory Syndrome Vaccine

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