关键词: 水稻；盐；ABA；表达谱；MYB

Abstract:

Transcription factors play important roles in plant development and response to stress in external environment. MYB family is a kind of plant transcription factor family with larger numbers and multiple functional diverse. This study took salt tolerant variety 177 and its recurrent parent IR64 as material, and treated them with salt and salt+ABA, then quantitative Real-time PCR was used to compare and analyze the expression profile of 43 MYB transcription factors. The results showed that 42% and 58% MYB transcription factors in IR64 and 177 were down-regulated in leave tissues under salt stress, while after exogenous ABA application the expression level of 61% (IR64) and 68% (177) those genes were significantly enhanced. This result indicated that exogenous ABA could change the expression patterns of MYB transcription factors under salt treatment, and then affected the rice salt tolerance. At the same time, this study identified 12 MYB transcription factors expressed differentially between 177 and IR64. The expression patterns of those genes might closely related to the different salt tolerance of these 2 varieties. This study provided theoretical referrances for further analyzing the mechanism of MYB genes in rice responce to salt tolerance, and the role of ABA in enhancing rice salt tolerance.

Key words: rice, salt, ABA, expression profile, MYB