关键词: 海带育苗, 低温循环水系统, 品种混杂, 孤雄生殖, 微卫星标记

Abstract: Seedling production of brown alga Saccharina japonica in China happens in recirculated cold seawater  to provide sufficiently large young plants for open sea cultivation when the seawater temperature drops below 20℃. In order to clarify whether there was a variety contamination problem in the sharing recirculated cold water system when more than one variety was produced in this investigation, a female gametophyte clone was used to test whether hybrid sporophytes could be obtained at the inlet of the water system and inside the seedling culture tanks, using parthenogenetic sporophytes as the control. Four microsatellite loci were used to further analyze the genotypes of the sporophytes. Results showed that the sporophytes obtained at the water inlet and inside the seedling production tanks were composed of alien genes indicating clearly the presence of variety contamination. Thus, this paper suggested that an independent water system was required to cultivate the parental plants in order to maintain purity and superior characteristics of each variety.

Key words: Saccharina japonica breeding, recirculated coled water system, varieties mixture, parthenogenesis, SSR loci