关键词: 春秋两季；不同地区；猪源耐药大肠杆菌；&beta, -内酰酶基因；16S rRNA甲基化酶基因

Abstract: In order to investigate β-lactam enzyme and 16S rRNA methylase genes in resistant swine E. coli isolated from different areas of Xinjiang  in spring and autumn and their coexistence. β-lactamase (blaTEM,blaCMY-2,blaLAP-1,blaKPC,blaSHV and blaOXA) and 16S rRNA methylase genes (armA and rmtB) were detected in 142 (spring) and 9 (autumn) swine resistant E. coli isolated from Karamay  area, 78 (spring) and 52 (autumn) swine resistant E. coli isolated from  Changji area  by PCR, respectively. And PCR products of the resistant genes  were sequenced. The results showed that blaTEM(142/142, 100%), blaOXA(16/142, 11.3%), blaCMY-2 (7/142, 4.9%) and blaLAP-1 (1/142, 0.7%) genes were mainly detected in swine resistant E. coli isolated from spring in Karamay area; blaTEM(9/9, 100%) and blaOXA(1/9, 11.1%) genes were mainly detected in swine resistant E. coli isolated from Karamay area in autumn; 16S rRNA methylase genes were not detected in swine resistant E. coli isolated from  Karamay area  in spring and autumn. β-lactamase genes as blaTEM(78/78, 100%), blaOXA(9/78, 11.5%), blaCMY-2 (3/78, 3.8%) and 16S rRNA methylase gene as rmtB (1/78, 1.3%) were mainly detected in swine resistant E. coli isolated from Changji area in spring ; blaTEM(52/52, 100%), blaOXA(4/52, 7.7%), blaCMY-2 (3/52, 5.8%) and blaSHV(1/52, 1.9%) genes were mainly detected in swine resistant E. coli isolated from  Changji area  in autumn, and 16S rRNA methylase genes were not detected. These results indicated that detection rate of blaTEM was 100% in swine resistant E. coli isolated from different areas  in spring and autumn. That detection rate of blaOXA in swine resistant E. coli isolated from different areas in spring was higher than that in autumn. Bacteria carry β-lactamase and 16S rRNA methylase genes were one of the main resistant reasons to antimicrobial drugs. So we should regulate the use of antibiotics, strengthen and monitor E. coli carrying the β-lactamase and 16S rRNA methylase genes.

Key words: spring and autumn, different areas, swine resistance E. coli, β-lactamase genes, 16S rRNA methylase genes