关键词: 黄瓜, 发根农杆菌, 乙酰丁香酮, 诱导率, 隶属函数法

Abstract: Taking 3 cultivars of cucumber “Jinyan 4”, “Xinjinchun 4” and “Yujiaolong” as experimental material, this study compared different conditions affecting induction rate of hairy root and comprehensively evaluated the induction abilities of hairy roots with A. rhizogenes (A4) among these 3 cucumber cultivers by the method of subordinate function. These studies would provide a high-efficiency system for the induction of agrobacterium rhizogenes in cucumber. Results showed that hairy roots were successfully induced in 3 cucumber cultivars, and they could grow normally after transferred. Analysis of different conditions showed that selections the stem with leaf as explant, 10 minutes as infection time, OD600=0.3 as microbial concentration and 10 mg/L as concentration of acetosyringone (As) were the optimized experiment conditions for induction rate of hairy roots. The order of induction ability on hair roots was “Yujiaolong”>“Jinyan 4”>“Xinjinchun 4” in these 3 cultivars of cucumber by subordinate function. It demonstrated that T-DNA on Ri plasmid of A4 had been integrated into the genome of nuclear cucumber genome successfully through molecular identification by PCR.

Key words: Cucumis sativus L., Agrobacterium rhizogenes, acetosyringone, induction rate, subordinate function