关键词: 猴头菇粗多糖, 响应曲面法, 提取工艺

Abstract: Applying Box-Behnken design and response surface methodology, this study optimized the extraction conditions for crude polysaccharide from Hericium erinaceus investigation. The extraction time, ratio of water to raw material and extraction temperature were selected as the key investigation factors. Box-Behnken design took the extraction rate as the response value. Design Expert software 7.0 was used to establish quadratic multinomial models fitting for experimental data. Contour overlay plot and response surface were delineated for the comparison of  interactions of each key factor. And then the optimal formulation was screened out and validated. The result showed that the optimal extraction parameters were as follows: extraction time 3.8 h, ratio of water to raw material 25 mL/g, extraction temperature 81℃. The results of validation test showed that the extraction rate was 3.09%,  basically met with the forecast value. Research results showed the model established by the response surface methodology had good predictability, and could rationally optimize the extracting technology for crude polysaccharide from Hericium erinaceus.

Key words: crudepolysaccharide from Hericium erinaceus, response surface methodology, extraction technology