关键词: 油菜籽粕, 芥子碱氯化盐, 血管内皮因子, 前列环素, 血栓素A2, 内皮素-1

Abstract: To investigate the antihypertensive effect and its influence on vascular endothelial growth factor of sinapine chlorate of rapeseed meal, the antihypertensive effect of sinapine chlorate on systolic blood pressure, diastolic blood pressure and mean arterial pressure was detected by arterial intubation into New Zealand white rabbits. The levels of prostacyclin (PGI2), thromboxane A2(TXA2) and endothelin-1 (ET-1) in serum were detected by enzyme linked immunosorbent assay (ELISA). The level of NO in serum was detected by nitrate reductase method. The results showed that sinapine chlorate could significantly decrease the systolic pressure, diastolic pressure and mean pressure in the range of 0.025~12.5 mg/kg. While with the dose increasing, the depressurization rate would be raised and holding time be prolonged. The results of ELISA showed that there was no significant effect on PGI2 and ET-1 in serum of experimental animals, and the level of TXA2 could be significantly reduced by the sinapine chlorate in 2.4 mg/kg and 9.6 mg/kg. Above results indicated sinapine chlorate had a significant antihypertensive effect, and the antihypertensive mechanism might be achieved by inhibiting levels of vasoconstriction factor TXA2.

Key words: rapeseed meal, sinapine chlorate, vascular endothelial growth factor, prostacyclin, thromboxane A2, endothelin-1