关键词: 水稻纹枯病菌, MAPK级联途径, 全基因组, 信号通路模型

Abstract: MAPK cascade signal pathway plays a key role in the growth, development, reproduction and pathogenicity of plant pathogenic fungi. According to the published genome-wide data of 5 rice sheath blight pathogen (Rhizoctonia solani), this paper identified the MAPK cascade signal pathway genes by bioinformatics, and predicted the MAPK signal pathways of rice sheath blight pathogen Rhizoctonia solani. The results of protein domain and motif analysis showed that the MAPK signal pathway genes of Rhizoctonia solani all contained serine/threonine protein kinase domain, and the gene sequences were conservatized. The results of multiple sequence alignment indicated that there were 11 MAPKKK genes, respectively belonging to Ste11, Bck1 and Ssk2; 10 MAPKK genes belonging to Ste7, Pbs2 and Mkk1; 12 MAPK genes were classified as Kss1/Fus3, Hog1, Slt2 and Ime2. Among the genome of the 5 rice sheath blight pathogen, MAPK cascade signal pathways of Fus3/Kss1-MAPK, Hog1-MAPK, Slt2-MAPK and Ime2-MAPK were found in R. solani (taxid: 456999) and AG-3 Rhs 1AP (taxid: 1086054). In genome of AG-1 IB (taxid: 1108050), there were Kss1/Fus3-MAPK, Hog1-MAPK, and Slt2-MAPK signal pathway. And there was only Hog1-MAPK signal pathway in genome of AG-8 WAC10335 (taxid: 1287689). While, 2 MAPKKK genes in genome of AG-1 IA (taxid: 983506) could not find complete signal pathway. Based on the above results, the signal pathway models of Kss1/Fus3, Hog1, Slt2 and Ime2 were predicted in R. solani. These results provided theoretical basis for further studying the function of MAPK family in R. solani.

Key words: Rhizoctonia solani, MAPK cascade signal pathway, whole-genome, signal path model