关键词: 铜藻, 岩藻黄素, 超声辅助, 工艺优化

Abstract: This paper studied the technology of fucoxanthin extraction from Sargassum horneri assisted by ultrasonic. The content of fucoxanthin was analyzed by high performance liquid chromatography (HPLC). Single factor experiments were conducted to investigate the factors affecting extraction rate of fucoxanthin, including solvent, ethyl alcohol concentration, ultrasound time, solid-liquid ratio, extraction temperature and extraction times. The optimized technology was obtained by orthogonal test. The optimum extraction technology included 90% ethanol concentration, 18 min ultrasound time, solid-liquid ratio 1 g∶5 mL, extraction temperature 45℃ and extract time twice. Under these conditions, the extraction rate of fucoxanthin was 0.292 5 mg/g FW (1.460 3 mg/g DW). The fucoxanthin content in fresh Sargassum horneri was very rich. The extraction rate of fucoxanthin with ethyl alcohol assisted by ultrasonic was high, and the method was easy and simple to handle.

Key words: Sargassum horneri, fucoxanthin, ultrasonic assisted, technology optimization