关键词: 抗病育种, 基因调控网络, 小鼠黑色素瘤, 功能异常CD8+ T 细胞, 特有基因

Abstract: Disease is a major threat to livestock and poultry health worldwide. Identifying key genes related to disease resistance has become a research hotpot in molecular breeding of disease resistance. To better understand the response mechanism of dysfunctional CD8+ T cell to immune therapy treatment, the gene regulatory network was constructed for the dysfunctional CD8+ T cells under PD1, IgG antibody treatments. There were 28, 17, 33 genes being identified in control, IgG and PD1 antibody treatment groups, respectively. Two transcription factors of Zfx and Zfhx3 expressed silencing under treatments of both IgG and PD1. The surface receptor of Raet1b expressed increasingly under treatment of PD1, which might recover dysfunctional CD8+ T cells. This research provided theoretical basis for gene exploring related to disease resistance breeding.

Key words: disease resistance breeding, gene regulatory network, mouse melanoma model, dysfunctional CD8+ T cell, specific gene