关键词: 杜仲, 愈伤组织, 增殖培养

Abstract: Eucommia ulmoides is one of the most valuable Chinese medicinal materials and the most promising gum source plants. In this paper, the culture system of callus induction and proliferation of Eucommia ulmoides was established by screening explants and optimizing the culture medium. The results showed that cotyledons, leaves and hypocotyls could induce callus, but the better explants were hypocotyls. The better basal medium for callus induction was B5, and the optimal phytohormones were NAA 1.0 mg/L and 2, 4-D 2.0 mg/L. The optimal phytohormone for callus proliferation culture were 6-BA 0.5 mg/L, NAA 0.5 mg/L and 2,4-D 1.0 mg/L. This paper established a method for callus culture of Eucommia ulmoides Oliv., which provided technical support for obtaining metabolites through cell culture.

Key words: Eucommia ulmoides, callus, proliferative culture