关键词: 玉米, 矮杆基因, 甲基磺酸乙酯, 突变体, BSR

Abstract: Exploring dwarf genes is very important for maize breeding to increase plant dense. In this study, a series of maize dwarf mutants were obtained by screening  mutant library, which was constructed from inbred line Zheng 58 treated with ethyl methylate (EMS). Height of the dwarf mutants decreased by 10%~45%, the spike height decreased by 10%~65%, and the number of internodes of some mutants was also changed. All of the dwarf mutants cell were shortened by 25%~75%. Height of dwarf mutants was not only decided by cell shorting, also by cells number. To isolate dwarf candidate genes of mutants, five mutants were selected to construct the F2 segregation population. Combining BSR high-throughput sequencing and bioinformatics analysis, candidate genes of five mutants were obtained. These results provided an effective analytical method  and new maize dwarf resource for dwarf gene isolation from mutants.

Key words: maize, dwarf gene, EMS, mutant, BSR