关键词: 微流控芯片, 多精入卵, 辅助生殖, 体外受精, 体外胚胎培养

Abstract: With the advantages of automation, high sensitivity and precision, microfluidic technology have been used to control complex and precise biological experiments, to ensure real-time and accurate acquisition of experimental phenomena. In this paper, the applications of microfluidic devices in IVM（in vitro maturation）, IVF（in vitro fertilization） and IVC（in vitro culture） of mammalian oocytes were reviewed, and the applications of microfluidic technology in mammalian embryology research were also summarized. At the same time, this paper proposed to apply the microfluidic technology to the IVF of porcine, aiming to solve and overcome the multiple spermatozoa in the IVF of porcine, and to provide a feasible solution for improving the IVF of porcine. At the same time, it provided new ideas for the optimization and improvement of human medical assisted reproductive technology.

Key words: microfluidic chip, polyspermy, assisted reproduction, in vitro fertilization, in vitro culture