旱盐交叉胁迫对甜高粱种子萌发和生理特性的影响

doi:10.13304/j.nykjdb.2019.0561

中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (2): 37-49.DOI: 10.13304/j.nykjdb.2019.0561

旱盐交叉胁迫对甜高粱种子萌发和生理特性的影响

王志恒,杨秀柳,邹芳,黄思麒,周吴艳,徐中伟,魏玉清*

北方民族大学生物科学与工程学院，国家民委生态系统模型及应用重点试验室，银川 750021

收稿日期:2019-07-10 出版日期:2021-02-15 发布日期:2019-08-16
通讯作者: 魏玉清 E-mail：weiyuqing@126.com
作者简介:王志恒 E-mail：wangzhiheng08@qq.com
基金资助:
国家自然科学基金项目（31060180）；
北方民族大学研究生创新项目(YCX19108)；
国家级大学生创新训练计划项目（201911407019）；
自治区级大学生创新训练计划项目（S201911407044）

Effects of Salt and Drought Cross Stress on Germination and Physiological Characteristics of Sweet Sorghum Seeds

WANG Zhiheng, YANG Xiuliu, ZOU Fang, HUANG Siqi, ZHOU Wuyan, XU Zhongwei, WEI Yuqing*

Key Laboratory of Ecosystem Modelling and Application of State Nationalities Affairs Commission, College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China

Received:2019-07-10 Online:2021-02-15 Published:2019-08-16

摘要/Abstract

摘要： 为探讨干旱与盐胁迫及其交互作用对甜高粱种子萌发及早期幼苗生长及生理特性的影响，以辽甜1号甜高粱为试验材料，采用PEG-6000（0、50、100 g·L^-1）和NaCl（0、75、150 mmol·L^-1）模拟不同程度的干旱胁迫和盐胁迫，研究单一胁迫及交互胁迫对甜高粱种子萌发特征和早期幼苗生理特性的影响。结果表明：单一胁迫或交叉胁迫均能抑制甜高粱种子的萌发指标和种苗转化指标，随着胁迫程度加深，早期幼苗的抗氧化物酶活性和可溶性蛋白、脯氨酸（proline，Pro）含量呈先升高后下降趋势，丙二醛（malondialdehyde，MDA）和谷胱甘肽（glutathione，GSH）含量逐渐升高。相比较单一胁迫，特定条件下的交互胁迫可促进甜高粱种苗转化和增强幼苗的抗氧化物酶活性，提高可溶性蛋白、Pro、MDA、GSH含量。双因素方差分析结果验证了单一胁迫对甜高粱种子萌发和幼苗生理的影响比旱盐交叉胁迫影响更大。隶属函数综合评价可知，甜高粱萌发期耐受性强弱为：交叉胁迫>NaCl胁迫> PEG-6000胁迫。旱盐交叉胁迫对甜高粱种子萌发及幼苗生理特性的影响并不是单一胁迫的简单叠加，与单一胁迫相比，旱盐交叉胁迫在一定程度上能够缓解盐胁迫对甜高粱种子萌发及幼苗生长生理的影响。

关键词: 甜高粱, 旱盐交叉胁迫, 种子萌发, 生理特性

Abstract: In order to study effects of cross stress induced by salt and drought on seed germination and physiological characteristics of early seedlings of sweet sorghum，Liaotian No.1 sweet sorghum seed was used as the test material. PEG-6000 (0, 50 and 100 g·L^-1) was used to simulate drought stress, and NaCl (0, 75 and 150 mmol·L^-1) was used to simulate salt stress. The effects of drought and salt stress on the germination characteristics of sweet sorghum seeds and physiological characteristics of early seedlings were studied. The results showed that: single or cross stress could inhibit the germination and seedling transformation of sweet sorghum seeds. With the deepening of stress, the antioxidant activity, soluble protein and proline (Pro) content of the seedlings showed an increasing and then decreasing trend, and the malondialdehyde (MDA) and glutathione (GSH) content increased gradually. Compared with single stress, interactive stress under specific conditions could promote the germination of sweet sorghum seeds, enhance the antioxidant activity of seedlings, and increase the content of soluble protein, Pro, MDA and GSH. The results of two-factor variance analysis showed that the effects of single stress on seed germination and seedling physiology of sweet sorghum were more greater than that of drought salt cross stress. According to the comprehensive evaluation of the membership function, sweet sorghum of the stress tested was found as follows: interaction stress> single PEG stress> single NaCl stress. The effects of drought salt cross stress on seed germination and physiological characteristics of sweet sorghum seedlings were not simply superimposed by single stress. Compared with single stress, drought salt cross stress could alleviate the effects of salt stress on seed germination and seedling growth physiology of sweet sorghum to a certain extent.

Key words: sweet sorghum, drought salt cross stress, seed germination, physiological characteristics

王志恒,杨秀柳,邹芳,黄思麒,周吴艳,徐中伟,魏玉清*. 旱盐交叉胁迫对甜高粱种子萌发和生理特性的影响[J]. 中国农业科技导报, 2021, 23(2): 37-49.

WANG Zhiheng, YANG Xiuliu, ZOU Fang, HUANG Siqi, ZHOU Wuyan, XU Zhongwei, WEI Yuqing*. Effects of Salt and Drought Cross Stress on Germination and Physiological Characteristics of Sweet Sorghum Seeds[J]. Journal of Agricultural Science and Technology, 2021, 23(2): 37-49.

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旱盐交叉胁迫对甜高粱种子萌发和生理特性的影响

Effects of Salt and Drought Cross Stress on Germination and Physiological Characteristics of Sweet Sorghum Seeds

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