铅、锌、镉胁迫对多年生黑麦草生长及生理生化特性的影响

doi:10.13304/j.nykjdb.2019.0752

中国农业科技导报 ›› 2021, Vol. 23 ›› Issue (3): 41-50.DOI: 10.13304/j.nykjdb.2019.0752

铅、锌、镉胁迫对多年生黑麦草生长及生理生化特性的影响

张茂,徐彦红,席溢*,裴应杰,黄本用,杨克超,李金孟

贵州大学动物科学学院, 贵阳 550025

收稿日期:2019-09-11 出版日期:2021-03-15 发布日期:2019-12-18
通讯作者: 席溢 E-mail：xyii2000@126.com
作者简介:张茂 E-mail：471078341@qq.com
基金资助:
贵州大学“SRT计划”项目（2016181）；
贵州省科技重大专项计划项目（黔科合重大专项字\[2016\]3002号）；
贵州省科技合作计划项目（黔科合LH字\[2016\]7450号）；
贵州大学“本科教学工程”建设项目（JG201915）

Effects of Pb^2+, Zn²⁺ and Cd²⁺ on Growth, Physiological and Biochemical Characteristics of Perennial Ryegrass

ZHANG Mao, XU Yanhong, XI Yi*, PEI Yingjie, HUANG Benyong, YANG Kechao, LI Jinmeng

College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2019-09-11 Online:2021-03-15 Published:2019-12-18

摘要/Abstract

摘要： 为了解多年生黑麦草对Pb、Zn、Cd的耐性和土壤重金属污染的修复潜力，采用砂培法研究铅（Pb）、锌（Zn）、镉（Cd）对多年生黑麦草生长发育及生理生化特性的影响。Pb²⁺设0 (CK)、500 (Pb₅₀₀)、1 000 (Pb_{1 000})、2 000 mg·kg^-1 (Pb_{2 000})四个处理，Zn²⁺设0 (CK)、500 (Zn₅₀₀)、1 000 (Zn_{1 000})、2 000 mg·kg^-1 (Zn_{2 000})四个处理，Cd ²⁺设0 (CK)、10 (Cd₁₀)、20 (Cd₂₀)、50 mg·kg^-1 (Cd₅₀)四个处理，测定不同浓度Pb ²⁺ 、Zn ²⁺ 、Cd ²⁺胁迫下多年生黑麦草的形态特征、叶绿素含量、抗氧化酶活性、膜脂过氧化产物含量、渗透调节物质含量，利用Fuzzy隶属函数法对这些指标进行综合评价。结果表明：①Pb ²⁺ 、Zn ²⁺ 、Cd ²⁺胁迫显著抑制多年生黑麦草的生长，Pb2 000、Zn2 000和Cd50三个处理对黑麦草的生物量、分蘖及根系生长的抑制作用最显著。②Pb ²⁺ 、Zn ²⁺ 、Cd ²⁺胁迫时，多年生黑麦草会通过增加植物的渗透调节物质和抗氧化酶活性减缓对植物的伤害。超氧化物歧化酶、过氧化物酶活性均随着Pb ²⁺ 、Zn ²⁺ 、Cd ²⁺胁迫浓度的增加呈现先增加后减少趋势；丙二醛和脯氨酸含量随着重金属浓度增加而增加；Pb500和Cd10处理的可溶性糖含量下降，而Zn500处理的可溶性糖含量增加。（3）多年生黑麦草对Pb ²⁺ 、Zn ²⁺ 、Cd ²⁺胁迫有一定的耐受性，不同处理的植株抗逆性由强到弱依次表现为：Zn₅₀₀> CK > Zn_{1 000}> Cd₁₀> Pb₅₀₀ > Zn_{2 000}> Cd₂₀ > Pb_{1 000} > Pb_{2 000}> Cd₅₀。研究结果为生态恢复提供理论支撑和数据支持。

关键词: 多年生黑麦草, 生理生化, 铅, 锌, 镉

Abstract: In order to understand the tolerance of perennial ryegrass to Pb, Zn and Cd and the remediation potential of heavy metal pollution in soil, sand culture method was used to study the effects of plumbum (Pb), zinc (Zn) and cadmium (Cd) on the growth, physiological and biochemical characteristics of perennial ryegrass. Pb²⁺ treatments with 0 (CK), 500 (Pb₅₀₀), 1 000 (Pb_{1 000}) and 2 000 mg·kg^-1 (Pb_{2 000}) concentrations, Zn²⁺ treatments with 0 (CK), 500 (Zn₅₀₀), 1 000 (Zn_{1 000}) and 2 000 mg·kg^-1 (Zn_{2 000})concentrations, Cd ²⁺ treatments with 0 (CK), 10 (Cd₁₀), 20 (Cd₂₀) and 50 mg·kg^-1 (Cd₅₀) concentrations were set up. The morphological characteristics, chlorophyll content, antioxidant enzymes activities, membrane lipid peroxidation product content and osmotic adjustment substances contents of perennial ryegrass under different concentrations of Pb²⁺, Zn²⁺ and Cd ²⁺ were determined, and these indexes were comprehensively evaluated by Fuzzy membership function method. The results showed that: ① Pb²⁺, Zn²⁺ and Cd ²⁺ stress significantly inhibited the growth of perennial ryegrass, and Pb_{2 000}, Zn_{2 000}and Cd₅₀ treatments had the most significant inhibition on biomass, tiller number and root growth of perennial ryegrass. ② Under the stress of Pb²⁺, Zn²⁺ and Cd ²⁺, perennial ryegrass slowed down the damage to plants by increasing osmotic adjustment substances and antioxidant enzymes activities of plants. The contents of superoxide dismutase and peroxidase were increased first and then decreased with the increase of Pb²⁺, Zn²⁺ and Cd ²⁺ concentrations. Malondialdehyde and proline contents were increased with the increase of Pb²⁺, Zn²⁺ and Cd ²⁺ concentrations. With the increase of Pb²⁺, Zn²⁺ and Cd ²⁺ concentrations, the soluble sugar contents of Pb500 and Cd10 treatments were decreased, while that of Zn500 treatment were increased. ③ Perennial ryegrass had certain tolerance to Pb²⁺, Zn²⁺ and Cd ²⁺, and the stress resistance of plants in all treatments showed as following: Zn₅₀₀> CK > Zn_{1 000}> Cd₁₀> Pb₅₀₀ > Zn_{2 000}> Cd₂₀ > Pb_{1 000} > Pb_{2 000}> Cd₅₀. The present research provided theoretical and data support for ecological restoration.

Key words: perennial ryegrass, physiology and biochemistry, plumbum, zinc, cadmium

张茂,徐彦红,席溢*,裴应杰,黄本用,杨克超,李金孟. 铅、锌、镉胁迫对多年生黑麦草生长及生理生化特性的影响[J]. 中国农业科技导报, 2021, 23(3): 41-50.

ZHANG Mao, XU Yanhong, XI Yi*, PEI Yingjie, HUANG Benyong, YANG Kechao, LI Jinmeng. Effects of Pb^2+, Zn²⁺ and Cd²⁺ on Growth, Physiological and Biochemical Characteristics of Perennial Ryegrass[J]. Journal of Agricultural Science and Technology, 2021, 23(3): 41-50.

[1]	胡杨, 李钢铁, 李星, 贾守义. 干旱胁迫对细穗柽柳幼苗生长和生理生化指标的影响[J]. 中国农业科技导报, 2021, 23(6): 43-50.
[2]	孙艳, 蒲文宣, 吴长征, 黄平俊, 孙铭雪, 宋德安, 刘来华. 植物响应亚适低温的生长发育及分子机制研究进展[J]. 中国农业科技导报, 2021, 23(5): 18-26.
[3]	郝正刚,赵会君,魏玉清*,曾周琦,王志恒. 甜高粱对镉胁迫的生理生化响应及镉富集研究[J]. 中国农业科技导报, 2021, 23(1): 30-42.
[4]	罗勇1,焦桂珍1,刘胜波2,魏跃伟1,邵惠芳1,贾宏昉1. 不同浓度镉对烟草幼苗生长发育及生长素相关基因表达的影响[J]. 中国农业科技导报, 2021, 23(1): 58-65.
[5]	王飞1,王建国2,李林1,刘登望1,万书波2,张昊1. 不同施肥模式对花生Ca、Zn吸收、积累及分配的影响[J]. 中国农业科技导报, 2020, 22(5): 166-173.
[6]	邹芳，杨秀柳，黄思麒，王志恒，魏玉清*. 外源亚精胺对干旱胁迫下甜高粱幼苗生长及生理生化指标的影响[J]. 中国农业科技导报, 2020, 22(4): 44-52.
[7]	刘师豆1,朱新萍1,2*,赵一1,王博言1,韩耀光1,杨贝贝1,卢智1,贾宏涛1,2. 棉秆炭减缓土壤中镉对水稻生长的胁迫效应[J]. 中国农业科技导报, 2020, 22(4): 139-146.
[8]	邹苗1,王月林2,李钢铁1*,冯斐1,麻云霞1,赵雨兴3,石麟3,任余燕3. 干旱胁迫对酸枣幼苗生理特性的影响[J]. 中国农业科技导报, 2020, 22(2): 65-72.
[9]	徐一兰,刘唐兴,付爱斌. 不同栽插方式对双季稻生理特性和产量的影响[J]. 中国农业科技导报, 2020, 22(12): 20-28.
[10]	刘朋飞1,闫筱筱1,杨欣玲2,杨永峰2,张洪映1,崔红1,王召军1*. 分泌型和非分泌型烟草叶面腺毛对镉胁迫的应答反应研究[J]. 中国农业科技导报, 2019, 21(6): 55-60.
[11]	徐琴1,2§,王孟1,3§,谢义梅4,陈甜甜1,周灵4,谭亚华5,石垒1,3,邢丹英1,3*. 施硒对水稻外观品质及籽粒硒、镉和砷含量的影响[J]. 中国农业科技导报, 2019, 21(5): 135-140.
[12]	张晓1,张环纬1,陈彪1,王凯悦1,周子方2,邵惠芳1,许自成1,黄五星1*. 外源硅及水杨酸对镉胁迫下烟草幼苗生长和生理特性的影响[J]. 中国农业科技导报, 2019, 21(3): 133-140.
[13]	刘慧颖1,2,乔宇1,石波1,彭晴1*. 不同形貌氧化锌微/纳米颗粒对食源性致病菌的抑菌研究[J]. 中国农业科技导报, 2018, 20(5): 140-147.
[14]	张杰,陈彪,马晓寒,郭豪,许自成*. 气体信号分子在植物干旱胁迫下的调控作用及机制的研究进展[J]. 中国农业科技导报, 2018, 20(12): 52-58.
[15]	景宇鹏1,2,连海飞1,李焕春1,史培1,杜超3,刘梅4,常新娟5. 玉米耐盐碱能力及评价指标筛选研究[J]. 中国农业科技导报, 2018, 20(11): 94-104.

铅、锌、镉胁迫对多年生黑麦草生长及生理生化特性的影响

Effects of Pb^2+, Zn²⁺ and Cd²⁺ on Growth, Physiological and Biochemical Characteristics of Perennial Ryegrass

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

铅、锌、镉胁迫对多年生黑麦草生长及生理生化特性的影响

Effects of Pb2+, Zn2+ and Cd2+ on Growth, Physiological and Biochemical Characteristics of Perennial Ryegrass

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

Effects of Pb^2+, Zn²⁺ and Cd²⁺ on Growth, Physiological and Biochemical Characteristics of Perennial Ryegrass